Syngeneic Studies

Experimental Design

• Subcutaneous engraftment into 6-8 week old mice of CT26 (BALB/cJ) backgrounds
• 5 study groups (n=10 per group):
  • Vehicle, positive control (JAX provides), and three test groups (2 compounds+1 combo, 3 compounds or other permutations)
  • Dosing up to 2x per week for up to 28 days
  • Body weight/clinical observations/tumor volume measurements up to 3x per week

Deliverables

• Study report
• Terminal blood
• Tumor collection

Add-ons

• FACS analysis (blood, spleen, lymph nodes, tumor)
• Histology

Example Study: Anti-Mouse PD1/CTLA4 in CT26 engrafted BALB/cJ

Mice: Female BALB/cJ at ~6 weeks of age

Cells: CT26 cells subcutaneously injected at 2.5x10⁶ cells per mouse

Dosing: Mice were grouped into four groups: IgG control (n=10), Anti PD-1 (n=9), Anti CTLA-4 (n=10), and Anti PD-1 +Anti CTLA-4 (n=10). Mice were IP dosed as follows:

Measurements: Body weight/clinical observations/tumor volume measurements 2X-3X per week

Duration: Mice were taken down when tumor volume reached 2000mm³ or when the tumor became ulcerated

Study Results: Anti-Mouse PD1/CTLA4 in CT26 Engrafted BALB/cJ – Tumor Volume