Jackson Laboratory

NSG™ Variants Portfolio

NSG™ mouse model variants are the most highly immunodeficient
mice and the models of choice for cancer xenograft modeling, stem
cell biology, humanized mice, and infectious disease research.

Comparison of NSG™ Mouse Model Variants

Strain Name	Model Benefits	Strain Considerations	Research Areas	Reference
NSG™ (005557) NOD scid gamma	Engrafts the widest range of solid and hematological cancers, including ALL and AML Most sensitive host for cancer stem cells when compared to NOD scid or nude mice Longer lifespan than NOD scid; supports long-term engraftment studies and capabilities; >89 weeks median	No thymic lymphomas, can be used for long & short-term experiments Sensitive to irradiation	N/A	Ishikawa et al., 2005 Shultz et al., 2005
NSG-SGM3 (013062) NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ	Increased CD4+ FoxP3+ regulatory T cell population after CD34+ humanization Enhances human myelopoiesis and terminal differentiation after CD34+ humanization Increased efficiency of engrafting human acute myeloid leukemia (AML) Supports higher levels of human myeloid cell engraftment	Compromised human stem cell regeneration Suppression of human erythropoiesis Reduction of human B-lymphopoiesis	Immunology Oncology	Nicolini et al., 2004 Wunderlich et al., 2010 Billerbeck et al., 2011 Miller, et al., 2013
NRG (007799) NOD Rag gamma	Long-term multilineage hematopoeitic stem cell repopulation similar to NSG mice Engrafts human PBMC without irradiation similar to NSG Engrafts a wide range of solid and hematological cancers Greater tolerance to genotoxic agents compared to NSG	No thymic lymphomas, can be used for long & short-term experiments Requires higher dose of irradiation to obtain human HSC engraftment The serum half-life of human IgG is greatly reduced in NSG-B2m due to defective FcRn function	Immunology	Pearson et al., 2008 Brehm et al., 2010 Maykel et al., 2014
NSG-A2 (009617) NOD.Cg-Mcph1^{Tg(HLA-A2.1)1Enge} Prkdc^scid Il2rg^tm1Wjl/SzJ	High engraftment of HLA-A2-restricted immune cells after CD34+ humanization Antibody responses improved in a Dengue virus infection model Allow HLA restriction of developing human CD8 cells in humanized models, and provide improved platforms for viral infection and vaccine models	No thymic lymphomas - can be used for long-term experiments Sensitive to irradiation	Immunology HIV and Infectious Disease	Shultz et al., 2010 Strowig et al., 2009 Jaiswal et al., 2012
NSG-HLA-A2/HHD (014570) NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(HLA-A/H2-D/B2M)1Dvs/SzJ	Enable functional CD4+ T cell responses to viral infection after CD34+ humanization Permit HLA restriction and enhance immune responses of human CD8 T cells Antibody responses improved in a Dengue virus infection model	No thymic lymphomas - can be used for long-term experiments Sensitive to irradiation	Immunology HIV and Infectious Disease	Shultz et al., 2010 Strowig et al., 2009 Jaiswal et al., 2012
NSG-DR1 (012479) NOD.Cg-Tg(HLA-DRA0101,HLA-DRB10101)1Dmz Prkdc^scid Il2rg^tm1Wjl/GckRolyJ	Useful for transplantation studies in the absence of xeno-GVHD	Reduced CD45+ cell engraftment compared to NSG	Immunology HIV and Infectious Disease	Covassin et al., 2011
NSG-Ab^o DR4 (017637) NOD.Cg-Prkdc^scid Il2rg^tm1Wjl H2-Ab1^b-tm1Doi Tg(HLA-DRB1)31Dmz/SzJ	Useful for transplantation studies in the absence of xeno-GVHD Develop allo-GVHD post-engraftment of DR4-negative CD4+ T cells Show enhanced antibody responses, and human CD4+ T cells interact more effectively with human antigen presenting cells.	Reduced CD45+ cell engraftment compared to NSG Higher proportion of mice with no CD45+ cell engraftment as compared to NSG	Immunology HIV and Infectious Disease	Covassin et al., 2011 Danner R et al., 2011
NSG B2m (010636) NOD.Cg-B2m^tm1Unc Prkdc^scid Il2rg^tm1Wjl/SzJ	Resistant to xeno-GVHD Useful for studying mechanisms for xeno-GVHD	N/A	HIV and Infectious Disease Transplant Research	Covassin et al., 2013 King et al., 2008
NSG-(K^bD^b)^null (023848) NOD.Cg-Prkdc^scid H2-K1^b-tm1Bpe H2-D1^b-tm1Bpe Il2rg^tm1Wjl/SzJ	Attenuated xeno-GVHD development post-Hu-PBMC transplantation High Hu-CD45+ cell engraftment Useful for studying mechanisms for xeno-GVHD	Reduced survival post Hu-CD34+ cell transplantation compared to NSG mice	HIV and Infectious Disease Transplant Research	Covassin et al., 2013
NSG-MHC I/II DKO (025216) NOD.Cg-Prkdc^scid H2-K1^b-tm1Bpe H2-Ab1^g7-em1Mvw H2-D1^b-tm1Bpe Il2rg^tm1Wjl/SzJ	Most resistant to xeno-GVHD of all NSG variants Extended human IgG half-life compared to B2m knockouts	Resistance to xeno-GVHD associated with reduced CD45+ cell expansion in vivo Radiation sensitive	Transplant Research	Brehm et al., 2019
NSG-Tg(Hu-IL15) (030890) NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(IL15)1Sz/SzJ	Increased abundance and function of human NK cells following CD34-humanization	Sensitive to irradiation	Immunology	Brehm et al., 2019
NBSGW (026622) NOD.Cg-Kit^W-41J Tyr⁺ Prkdc^scid Il2rg^tm1Wjl/ThomJ	Human CD34 cells engraft effectively without preconditioning irradiation Longer lifespan than other immunodeficient Kit mutants Increased human erythroid cells (bone marrow)	Although irradiation is not required for stem cell engraftment, NBSGW mice likely share the same sensitivity to genotoxic agents as other NSG strains	Immunology	McIntosh et al., 2015
NSG-PiZ (028842) NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(SERPINA1*E342K)#Slcw/SzJ	Engrafts human and allogeneic mouse hepatocytes without rejection	Shares the same sensitivity to genotoxic agents as other NSG strains	Transplant Research	Borel et al., 2017
NSG-TLR4 KO (033704) NOD.Cg-Tlr4^lps-del Prkdc^scid Il2rg^tm1Wjl/SzJ	Residual mouse innate immune system cannot respond to Tlr4 agonists After humanization, NSG-Tlr4 KO are ideal for studying human TLR4-specific responses against tumors	Shares the same sensitivity to genotoxic agents as other NSG strains	Transplant Research	Aryee et al., 2019