LOCUS       Exported               10248 bp DNA     linear   UNA 10-JAN-2023
DEFINITION  glucosylceramidase beta [Source:HGNC Symbol;Acc:HGNC:4177].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2023 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     gene ENSG00000177628
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
     gene            1..10248
                     /label=GBA
                     /note="gene ENSG00000177628
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..9832
                     /label=GBA-204
                     /note="primary transcript ENST00000428024"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 30..10248
                     /label=GBA-201
                     /note="primary transcript ENST00000327247"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 64..6487
                     /label=GBA-213
                     /note="primary transcript ENST00000493842
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 78..6136
                     /label=GBA-209
                     /note="primary transcript ENST00000473570
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 94..5065
                     /label=GBA-207
                     /note="primary transcript ENST00000467918
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3425..10141
                     /label=GBA-203
                     /note="primary transcript ENST00000427500"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3451..10248
                     /label=GBA-202
                     /note="primary transcript ENST00000368373"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 3457..4740
                     /label=GBA-208
                     /note="primary transcript ENST00000470104
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3469..9684
                     /label=GBA-211
                     /note="primary transcript ENST00000484489
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(3588..3614,3983..4070,4623..4814,4938..5084,6050..6183,
                     6394..6566,7122..7359,8231..8455,8856..9019,9389..9505,
                     9600..9705)
                     /codon_start=1
                     /label=GBA-202
                     /note="coding sequence ENSP00000357357"
                     /note="This feature has 11 segments:
                         1: 3588 .. 3614 / #993366 / ENSE00001890492
                         2: 3983 .. 4070 / #993366 / ENSE00003469059
                         3: 4623 .. 4814 / #993366 / ENSE00003675620
                         4: 4938 .. 5084 / #993366 / ENSE00003499798
                         5: 6050 .. 6183 / #993366 / ENSE00003618264
                         6: 6394 .. 6566 / #993366 / ENSE00003562842
                         7: 7122 .. 7359 / #993366 / ENSE00003488376
                         8: 8231 .. 8455 / #993366 / ENSE00001231060
                         9: 8856 .. 9019 / #993366 / ENSE00003506590
                        10: 9389 .. 9505 / #993366 / ENSE00003644399
                        11: 9600 .. 9705 / #993366 / ENSE00001917720"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGL
                     LLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMA
                     SCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPT
                     WLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLS
                     GYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEA
                     AKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
                     MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYH
                     LGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVG
                     FLETISPGYSIHTYLWRRQ"
     CDS             join(3588..3614,3983..4070,4623..4814,4938..5084,6050..6183,
                     6394..6566,7122..7359,8231..8455,8856..9019,9389..9505,
                     9600..9705)
                     /codon_start=1
                     /label=GBA-201
                     /note="coding sequence ENSP00000314508"
                     /note="This feature has 11 segments:
                         1: 3588 .. 3614 / #993366 / ENSE00003612715
                         2: 3983 .. 4070 / #993366 / ENSE00003469059
                         3: 4623 .. 4814 / #993366 / ENSE00003675620
                         4: 4938 .. 5084 / #993366 / ENSE00003499798
                         5: 6050 .. 6183 / #993366 / ENSE00003618264
                         6: 6394 .. 6566 / #993366 / ENSE00003562842
                         7: 7122 .. 7359 / #993366 / ENSE00003488376
                         8: 8231 .. 8455 / #993366 / ENSE00001231060
                         9: 8856 .. 9019 / #993366 / ENSE00003506590
                        10: 9389 .. 9505 / #993366 / ENSE00003644399
                        11: 9600 .. 9705 / #993366 / ENSE00001917720"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGL
                     LLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMA
                     SCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPT
                     WLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLS
                     GYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEA
                     AKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
                     MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYH
                     LGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVG
                     FLETISPGYSIHTYLWRRQ"
     CDS             join(3588..3614,3983..4070,4623..4814,6050..6183,6394..6566,
                     7122..7359,8231..8455,8856..9019,9389..9505,9600..9705)
                     /codon_start=1
                     /label=GBA-203
                     /note="coding sequence ENSP00000402577"
                     /note="This feature has 10 segments:
                         1: 3588 .. 3614 / #993366 / ENSE00002249126
                         2: 3983 .. 4070 / #993366 / ENSE00003469059
                         3: 4623 .. 4814 / #993366 / ENSE00003675620
                         4: 6050 .. 6183 / #993366 / ENSE00003618264
                         5: 6394 .. 6566 / #993366 / ENSE00003562842
                         6: 7122 .. 7359 / #993366 / ENSE00003488376
                         7: 8231 .. 8455 / #993366 / ENSE00001231060
                         8: 8856 .. 9019 / #993366 / ENSE00003506590
                         9: 9389 .. 9505 / #993366 / ENSE00003644399
                        10: 9600 .. 9705 / #993366 / ENSE00002305450"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGI
                     GYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVS
                     LLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTA
                     ENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHW
                     AKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQ
                     SVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKD
                     TFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDV
                     PLTIKDPAVGFLETISPGYSIHTYLWRRQ"
     prim_transcript 4738..8304
                     /label=GBA-214
                     /note="primary transcript ENST00000497670
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(4769..4814,4938..5084,6050..6183,6394..6566,7122..7359,
                     8231..8455,8856..9019,9389..9505,9600..9705)
                     /codon_start=1
                     /label=GBA-204
                     /note="coding sequence ENSP00000397986"
                     /note="This feature has 9 segments:
                        1: 4769 .. 4814 / #993366 / ENSE00003578873
                        2: 4938 .. 5084 / #993366 / ENSE00003499798
                        3: 6050 .. 6183 / #993366 / ENSE00003618264
                        4: 6394 .. 6566 / #993366 / ENSE00003562842
                        5: 7122 .. 7359 / #993366 / ENSE00003488376
                        6: 8231 .. 8455 / #993366 / ENSE00001231060
                        7: 8856 .. 9019 / #993366 / ENSE00003506590
                        8: 9389 .. 9505 / #993366 / ENSE00003644399
                        9: 9600 .. 9705 / #993366 / ENSE00003711050"
                     /translation="MELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNI
                     LALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEE
                     DTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWAR
                     YFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLAN
                     STHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHR
                     LFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPE
                     GGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAV
                     ALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ"
     prim_transcript 5809..6768
                     /label=GBA-205
                     /note="primary transcript ENST00000460156
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 5991..8518
                     /label=GBA-212
                     /note="primary transcript ENST00000491081
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 8241..9739
                     /label=GBA-210
                     /note="primary transcript ENST00000478472
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     8580..8603
                     /label=PCR Reverse
                     /note="color: black; sequence: GGGGAACCATGATTCCCTATCTTC; 
                     added: 2023-01-10"
     primer_bind     8580..8603
                     /label=Sanger Sequencing
                     /note="color: black; sequence: GGGGAACCATGATTCCCTATCTTC; 
                     added: 2023-01-10"
     prim_transcript 8865..10247
                     /label=GBA-206
                     /note="primary transcript ENST00000464536
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    8937..9011
                     /label=Donor Template WT -> SNV
                     /note="color: #00ffff"
     primer_bind     complement(8937..9011)
                     /label=Donor Template WT -> SNV
                     /note="color: black; sequence: 
                     GCCAAGGTGGTAGAACATGGGCTGTTTGTAAAACGTGACCTTGGTGATGTCTACAATGA
                     TGGGACTGTCGACAAA; added: 2023-01-10"
     misc_feature    8968..8970
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    8971..8990
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     primer_bind     complement(8971..8990)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CTGTTTGTAAAACGTGTCCT; added:
                     2023-01-10"
     misc_feature    8974
                     /label=SNV
                     /note="WT = A
                     SNV = T"
                     /note="color: #ffcc00"
     primer_bind     complement(9284..9303)
                     /label=PCR Forward
                     /note="color: black; sequence: CCCATAACTCCTGCAGAGGC; added:
                     2023-01-10"
ORIGIN
        1 ctcttttcca atccaggtcc cgccccgact ccccagggct gcttttctcg cggctgcggg
       61 tggtcgggct gcatcctgcc ttcagagtct tactgcgcgg ggccccagtc tccagtcccg
      121 cccaggcgcc tttgcaggct gcggtgggat ttcgttttgc ctccggttgg ggctgctgtt
      181 tctcttcgcc gacggtaggc gtaatgaata tttcgacctt tggatcttag ctgtcccctc
      241 cctgcgttcg cacttaacct ttttcaccat tattattatt attgttatta ttattatttt
      301 ttgagggagt ctcgccctgt cgcccaggct ggagtgtaat ggcgccttct tggctcactg
      361 caacctccgc ctcccgggtt caggcgattc tccgacctca gcctcccaag tacgtgggat
      421 tacaggcacc cgccaccacg cacggctaat tttttgtatc ttttagtaga gacggggttt
      481 caccatgttg gtcaggctgg tctccaattc ctgacctcgt gatccgcccg cctcggcctg
      541 ccaaacagct gtgattatag gcgtgagcca ccgcgcccgg ccaaccatca ttattatttt
      601 taacggtaag gatggtcaga ttttactaat gaagaagaga ttataaaatc ttcaagtctt
      661 tatatccact tgctttttga ggggtggagt gggaagaagg ttatgtaatt catacgttct
      721 tcagacatgt gacaaacatt cacggagccc ggcgacgagc gtcggggttg ggattcgcac
      781 tggagctgca gatgggtgcc aggatggact ggtccctacc ctccgcttga acctaggagg
      841 cggaggttgc agtgaaccga gatcgtgcca ctgcactcca gcctgggtga cagagatact
      901 ccgtctcaaa aaaaaaaaca aaacaaaaaa caagcggact gggcgcagtg cctcaccctg
      961 taatcccagc actttgcaaa gccaaggcgg gaggatcctt tgagtttagg agtttgagac
     1021 caacctgcgc aacacagtaa gaccccgtct ctacaaaaaa tacagaaatt agccaggtgt
     1081 ggtggtgtgc gcctatagtc ccagctattc tggaggctga ggtgggagga ttgcttattc
     1141 tggaggcaga ggttgcactg agccgaaatc aagctactac actccatcca gggcaacata
     1201 cggagaccct gtctcaaaca aacaaacaaa aaattgctca gtacctggcc aaaaaagaag
     1261 aggctcacta tgcagagggg aagtggaagg agatgtttgg acttctaaac tcaatagagc
     1321 aggagaggca aatgtagaat gtgctcagga aatatctgtg agatgaatga acttgaggga
     1381 agtaaggtac tagatattac ctgccctacc cagaacaaat cctgtgcaat gtttccttga
     1441 aaagtgagaa gtctggaagg ggtggctact gacatagtga agcaactagt tcaattctac
     1501 aacttgacag ctacccctgt gccaggctat ctacgaggat acttagaatg cataagacat
     1561 tccttcaagg aactccagga acagaggcct gacatgttgc aatgtttagt gtcaagcagt
     1621 gtactagaga cacattatca cactcaaacc tcacaacaat tctgtgaggt aggagttatc
     1681 actccccttt tatagatgaa acagaggctt agagtgattg atttattgaa agtcaaacag
     1741 ccagtaaatg gtgtagccag gattccaaac ttgctgtctc actgagactg tacttaatta
     1801 ctggagggac cgggtgtggt ggctcattgc tataatccca acaccttggg aggctgaggc
     1861 tggtggatca cctgaggtca ggggttcgag accagcctgg ccaacatggt gaaaccccat
     1921 ctctactaaa aatacaaaaa ttagctgggc atggtggtgg gctcctgtaa tcccagctac
     1981 tcaggaggct gaggcagggc aattgcttga gccgagatca cactgcactc cagcctgggc
     2041 aacagggcaa gactctgtct caaaaccaaa aaaaaaaaaa ttactggagg aacctagaag
     2101 aagaaatgat caattttgct tggagtgtat ctagaaagac ttcactgaga tcatttaaag
     2161 aacaaaaagg atggctgggg tccagcgcag tggctcatgc ctgtaatccc agcactttcg
     2221 gataccaagg cagcagatca cctgaggtcc agagtttcag accagcctgg ccaacatagt
     2281 gaaaccccat ctctactaaa aataaaaaaa ttagctgagc atgttggagg gcacctgtaa
     2341 tcccagctac ttgggaggct gaggcaggag aatcactcga acccaggagg tggaggttgc
     2401 agtgagccaa gatcacgcca ctgcactcca gcctgggcaa cagagtgaga ctctgtctca
     2461 aaaaacaaca acaacaaaaa atacaaacaa gagacaagta gttcccaggt gcctaccaag
     2521 tggtcaggca ctgcacttac ctcactgact gcagtaacca ccctttgagg ttgtggcatt
     2581 gcctccattt tccaggcaag gaaatgggct gagagctggg attagtcagg tcatgactgt
     2641 gtgtgccact cccgctaaat ctcatttgat gtggttcatg aggccacacc atggacagct
     2701 tcctccttgt gtccactgag gatatggctt tgtacaacac tttggttttt gaacgacttt
     2761 acaaacctcc ctgtcttgtg aggaaggaag aacagttatt accatctgca tctgatgatg
     2821 aaacaaggga cgctgcagag gagccgcact gaccactccc tccctccagt cctgtcatcc
     2881 cactgccagt gtcccaccct cttgtgccct gcacttcact ggctaataac ccccctcact
     2941 ttttcctctg tgaagccatc ctggataatt ccccacccac gaatggtccc tcctcatctc
     3001 agagagctct ccatgcacac ctgttaccgt ttctgtcttt atctgtaaat atctgtgtgt
     3061 ctgacttcca tgcctcacac acctctatag ggcaaagact gtcttaaaca tcttggtagt
     3121 gtcagtattt tgcacagtga agtttttttt tttaaattat atcagcttta tttgtacctt
     3181 tttgacattt ctatcaaaaa agaagtgtgc ctgctgtggt tcccatcctc tgggatttag
     3241 gagcctctac cccattctcc atgcaaatct gtgttctagg ctcttcctaa agttgtcacc
     3301 catacatgcc ctccagagtt ttatagggca tataatctgt aacagatgag aggaagccaa
     3361 ttgcccttta gaaatatggc tgtgattgcc tcacttcctg tgtcatgtga cgctcctagt
     3421 catcacatga cccatccaca tcgggaagcc ggaattactt gcagggctaa cctagtgcct
     3481 atagctaagg caggtacctg catccttgtt tttgtttagt ggatcctcta tccttcagag
     3541 actctggaac ccctgtggtc ttctcttcat ctaatgaccc tgaggggatg gagttttcaa
     3601 gtccttccag agaggtaaga gagagagctc ccaatcagca ttgtcacagt gcttctggaa
     3661 tcctggcact ggaatttaat gaatgacaga ctctctttga atccagggcc atcatggctc
     3721 tttgagcaag gcacagatgg agggaggggt cgaagttgaa atgggtggga agagtggtgg
     3781 ggagcatcct gatttggggt gggcagagag ttgtcatcag aagggttgca gggagagctg
     3841 cacccaggtt tctgtgggcc ttgtcctaat gaatgtggga gaccgggcca tgggcaccca
     3901 aaggcagcta agccctgccc aggagagtag ttgaggggtg gagaggggct tgcttttcag
     3961 tcattcctca ttctgtcctc aggaatgtcc caagcctttg agtagggtaa gcatcatggc
     4021 tggcagcctc acaggattgc ttctacttca ggcagtgtcg tgggcatcag gtgagtgagt
     4081 caaggcagtg gggaggtagc acagagcctc ccttctgcct catagtcctt tggtagcctt
     4141 ccagtaagct ggtggtagac ttttagtagg tgctcaataa atccttttga gtgactgaga
     4201 ccaactttgg ggtgaggatt ttgttttttt tcttttgaaa cagagtctta ctctgttgcc
     4261 tgggctggag tgcagtggtg caattttggc tcattccaac ctctgcctcc cagattcaag
     4321 cgattctctt gcttcagctt cccaggtagc tgggattaca ggcggccacc actacgccca
     4381 gctaattttt gtatttttag tagagacggg gtttcaccat gctggcaagg caggtctcaa
     4441 actcctcacc tcaggtgatc cgcccacctc ggcctcctaa agtgctagga ttacaggtgt
     4501 gagcccctgc gcccggccaa ggggtgagga attttgaaac cgtgttcagt ctctcctagc
     4561 agatgtgtcc attctccatg tcttcatcag acctcactct gcttgtactc cctccctccc
     4621 aggtgcccgc ccctgcatcc ctaaaagctt cggctacagc tcggtggtgt gtgtctgcaa
     4681 tgccacatac tgtgactcct ttgacccccc gacctttcct gcccttggta ccttcagccg
     4741 ctatgagagt acacgcagtg ggcgacggat ggagctgagt atggggccca tccaggctaa
     4801 tcacacgggc acaggtaacc attacacccc tcaccccctg ggccaggctg ggtcctccta
     4861 gaggtaaatg gtgtcagtga tcaccatgga gtttcccgct gggtactgat acccttattc
     4921 cctgtggatg tcctcaggcc tgctactgac cctgcagcca gaacagaagt tccagaaagt
     4981 gaagggattt ggaggggcca tgacagatgc tgctgctctc aacatccttg ccctgtcacc
     5041 ccctgcccaa aatttgctac ttaaatcgta cttctctgaa gaaggtgagg aggaagggga
     5101 caagatgaca tagagccatt gaaacttttc gtttttcttt tcttttttta aaattttttt
     5161 gaggcagaat ctcactctgc ccattctgtc ggcgagacag gagtgcagtg gtgtgatctc
     5221 ccctcacagc aacctctgcc tcccaggcta tagtgattct cctgcctcag cctcctgagt
     5281 agctggaatt ataggcgtgc gccactacca cctggctaat ttttgtattt ttagtagaga
     5341 cagggtttca tcatgttgac caggctagtc ttaaactcct gacctcaaat gatatacctg
     5401 ccttggcctc ccgaagtgct ggaattacaa gtgtgagcca ccgagcccag cagacacttt
     5461 tcttttttct tttttttttt ttgagacaga gtctcgcact gtcacccagg ctggagtgca
     5521 gtggcacaat ctcagctcac tgcaacctcc acctcccggg ttcaggtgat tctcctgtct
     5581 cagcctctcg agtacctggg attacaggtg cctgccacca cgcccggcta attttttgta
     5641 tttttagtag agacagggtt tcactatgtt ggccaggatg attgcgaact cctgacctcg
     5701 tgatctgccc acatcggcct cccaaagtgc tgggattaca tgcgtgagcc actgacactt
     5761 ttctttgccc tttctttgga ccctgacttc tgcccatccc tgacatttgg ttcctgtttt
     5821 aatgccctgt gaaataagat ttcaccgcct atcatctgct aactgctacg gactcaggct
     5881 cagaaaggcc tgcgcttcac ccaggtgcca gcctccacag gttccaaccc aggagcccaa
     5941 gttccctttg gccctgactc agacactatt aggactggca agtgataagc agagtcccat
     6001 actctcctat tgactcggac taccatatct tgatcatcct tttctgtagg aatcggatat
     6061 aacatcatcc gggtacccat ggccagctgt gacttctcca tccgcaccta cacctatgca
     6121 gacacccctg atgatttcca gttgcacaac ttcagcctcc cagaggaaga taccaagctc
     6181 aaggtaggca ttctagcttt ttcaggccct gagggccctg atgtctgggg gttgagaaac
     6241 tgtagggtag gtctgcttgt acagacattt tgtcccctgc tgttttgtcc tgggggtggg
     6301 agggtggagg ctaatggctg aaccggatgc actggttggg ctagtatgtg ttccaactct
     6361 gggtgcttct ctcttcacta cctttgtctc tagatacccc tgattcaccg agccctgcag
     6421 ttggcccagc gtcccgtttc actccttgcc agcccctgga catcacccac ttggctcaag
     6481 accaatggag cggtgaatgg gaaggggtca ctcaagggac agcccggaga catctaccac
     6541 cagacctggg ccagatactt tgtgaagtaa gggatcagca aggatgtggg atcaggactg
     6601 gcctcccatt tagccatgct gatctgtgtc ccaaccctca acctagttcc acttccagat
     6661 ctgcctgtcc tcagctcacc tttctacctt ctgggccttt cagccttggg cctgtcaatc
     6721 ttgcccactc catcaggctt cctgttctct cggtctggcc cactttcttt ttatttttct
     6781 tctttttttt ttttttgaga aggagtctct ctctctgtca cccaggctgg agtgctgtgg
     6841 cgccatcttc actcactgta acctctgcct cctgagttca agcaattctc ctgcctcagc
     6901 cttccaagta gctgggatta taggcgcctg ccaccaggcc cagctgattt ttctattttt
     6961 agtagagacg gggtttcgcc aggctgttct cgaactcctg aactcaagtg atccacctgc
     7021 ctcggcttcc caaagtgctg ggattacagg tgtgagccac cacacccagc tggtctggtc
     7081 cactttcttg gccggatcat tcatgacctt tctcttgcca ggttcctgga tgcctatgct
     7141 gagcacaagt tacagttctg ggcagtgaca gctgaaaatg agccttctgc tgggctgttg
     7201 agtggatacc ccttccagtg cctgggcttc acccctgaac atcagcgaga cttcattgcc
     7261 cgtgacctag gtcctaccct cgccaacagt actcaccaca atgtccgcct actcatgctg
     7321 gatgaccaac gcttgctgct gccccactgg gcaaaggtgg taaggcctgg acctccatgg
     7381 tgctccagtg accttcaaat ccagcatcca aatgactggc tcccaaactt agagcgattt
     7441 ctctacccaa ctatggattc ctagagcacc attcccctgg acctccaggg tgccatggat
     7501 cccacagttg tcgcttgaaa cctttctagg ggctgggcga ggtggctcac tcatgcaaac
     7561 ccagcacttt gggaagccga ggcgggtgat cacctgaggt caggagttta agaccaccct
     7621 ggccaacgtg ttgaaaccct gtgtctacta aaatacaaaa aaaaaaaatt atctgggcat
     7681 gatggtgggt gtctgtaatc ccagctactc aggaggctga gaagggagaa tcagttgaac
     7741 ccgggagatg gtggttgcgg tgagccgaga tcgcgccact gcactccagc ctgggaggct
     7801 gagcgagact ccatctcgaa acaaaacaaa acaaaactat ctaggctggg ggtggtggtt
     7861 catgtatgta tgtgtatata catatatatg tgtttatatg tatatatata tacacacaca
     7921 cacatacata cacacacata cacacacaaa ttagctgggt gtggcacccg tgtagtccca
     7981 gctactcagg aggctaatgt gggaggatca gttgacccta ggaagtcaag gctgcagtga
     8041 gtcgtgattg cgccactgta ctccagcccg agtgacagag tgacatcctg tctcaaaaac
     8101 aaaaaaaaat ctccccaaac ctctctagtt gcattcttcc cgtcacccaa ctccaggatt
     8161 cctacaacag gaactagaag ttccagaagc ctgtgtgcaa ggtccaggat cagttgctct
     8221 tcctttgcag gtactgacag acccagaagc agctaaatat gttcatggca ttgctgtaca
     8281 ttggtacctg gactttctgg ctccagccaa agccacccta ggggagacac accgcctgtt
     8341 ccccaacacc atgctctttg cctcagaggc ctgtgtgggc tccaagttct gggagcagag
     8401 tgtgcggcta ggctcctggg atcgagggat gcagtacagc cacagcatca tcacggtaag
     8461 ccaccccagt ctcccttcct gcaaagcaga cctcagacct cttactagtt tcaccaaaga
     8521 ctgacagaag cccttcctgt ccagctttcc ccagctagcc tgcccttttg agcaactctg
     8581 gggaaccatg attccctatc ttccctttcc ttcacaggtc tgcacacctc attgcccctt
     8641 ttgcaactac tgaggcactt gcagctgcct cagacttctc agctcccctt gagatgcctg
     8701 gatcttcaca cccccaactc cttagctact aaggaatgtg cccctcacag ggctgaccta
     8761 cccacagctg cctctcccac atgtgaccct tacctacact ctctggggac ccccagtgtt
     8821 gcgcctttgt ctctttgcct ttgtccttac cctagaacct cctgtaccat gtggtcggct
     8881 ggaccgactg gaaccttgcc ctgaaccccg aaggaggacc caattgggtg cgtaactttg
     8941 tcgacagtcc catcattgta gacatcacca aggacacgtt ttacaaacag cccatgttct
     9001 accaccttgg ccacttcagg tgagtggagg gcgggcaccc ccattccata ccaggcctat
     9061 catctcctac atcggatggc ttacatcact ctacaccacg agggagcagg aaggtgttca
     9121 gggtggaacc tcggaagagg cacacccatc cccttttgca ccatggaggc aggaagtgac
     9181 taggtagcaa cagaaaaccc caatgcctga ggctggactg cgatgcagaa aagcagggtc
     9241 agtgcccagc agcatggctc caggcctaga gagccagggc agagcctctg caggagttat
     9301 ggggtgggtc cgtgggtggg tgacttctta gatgagggtt tcatgggagg taccccgagg
     9361 gactctgacc atctgttccc acattcagca agttcattcc tgagggctcc cagagagtgg
     9421 ggctggttgc cagtcagaag aacgacctgg acgcagtggc actgatgcat cccgatggct
     9481 ctgctgttgt ggtcgtgcta aaccggtgag ggcaatggtg aggtctggga agtgggctga
     9541 agacagcgtt gggggccttg gcaggatcac actctcagct tctcctccct gctccctagc
     9601 tcctctaagg atgtgcctct taccatcaag gatcctgctg tgggcttcct ggagacaatc
     9661 tcacctggct actccattca cacctacctg tggcgtcgcc agtgatggag cagatactca
     9721 aggaggcact gggctcagcc tgggcattaa agggacagag tcagctcaca cgctgtctgt
     9781 gactaaagag ggcacagcag ggccagtgtg agcttacagc gacgtaagcc caggggcaat
     9841 ggtttgggtg actcactttc ccctctaggt ggtgccaggg gctggaggcc cctagaaaaa
     9901 gatcagtaag ccccagtgtc cccccagccc ccatgcttat gtgaacatgc gctgtgtgct
     9961 gcttgctttg gaaactgggc ctgggtccag gcctagggtg agctcactgt ccgtacaaac
    10021 acaagatcag ggctgagggt aaggaaaaga agagactagg aaagctgggc ccaaaactgg
    10081 agactgtttg tctttcctgg agatgcagaa ctgggcccgt ggagcagcag tgtcagcatc
    10141 agggcggaag ccttaaagca gcagcgggtg tgcccaggca cccagatgat tcctatggca
    10201 ccagccagga aaaatggcag ctcttaaagg agaaaatgtt tgagccca
//
