LOCUS       INK2J00102R_PFN1        3910 bp DNA     linear   UNA 22-FEB-2024
DEFINITION  profilin 1 [Source:HGNC Symbol;Acc:HGNC:8881].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Apr 4, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000108518
FEATURES             Location/Qualifiers
     source          1..3910
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..3410
                     /label=PFN1
                     /note="gene ENSG00000108518
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..2369
                     /label=PFN1-202
                     /note="primary transcript ENST00000572383"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(43..118,507..799,2242..2369)
                     /codon_start=1
                     /label=PFN1-202
                     /note="coding sequence ENSP00000460363"
                     /note="This feature has 3 segments:
                        1:   43 ..  118 / #993366 / ENSE00002633594
                        2:  507 ..  799 / #993366 / ENSE00002668429
                        3: 2242 .. 2369 / #993366 / ENSE00002678014"
                     /translation="MHSRPRRRRRRGHTRRRPPVLPFGGGSGGARAAAQRAEPRSGRQR
                     VPRALRLPPPASRGSSSPVRGPSSSAESSPSSSAMAGWNAYIDNLMADGTCQDAAIVGY
                     KDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEF
                     SM"
     prim_transcript 532..3410
                     /label=PFN1-201
                     /note="primary transcript ENST00000225655"
                     /note="color: #ffcc00; direction: RIGHT"
     CDS             join(668..799,2242..2434,3065..3162)
                     /codon_start=1
                     /label=PFN1-201
                     /note="coding sequence ENSP00000225655"
                     /note="This feature has 3 segments:
                        1:  668 ..  799 / #993366 / ENSE00001324890
                        2: 2242 .. 2434 / #993366 / ENSE00000676461
                        3: 3065 .. 3162 / #993366 / ENSE00001488299"
                     /translation="MAGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITP
                     AEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVT
                     KTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY"
     prim_transcript 1593..3395
                     /label=PFN1-203
                     /note="primary transcript ENST00000574872"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(2218..2434,3065..3162)
                     /codon_start=1
                     /label=PFN1-203
                     /note="coding sequence ENSP00000465019"
                     /note="This feature has 2 segments:
                        1: 2218 .. 2434 / #993366 / ENSE00002681343
                        2: 3065 .. 3162 / #993366 / ENSE00002644033"
                     /translation="MGAWFLFQPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQD
                     GEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY
                     "
     primer_bind     2804..2828
                     /label=PCR Forward
                     /note="color: black; sequence: CACATGAATCAACAGTCTATGAGCC; 
                     added: 2024-02-22"
     prim_transcript complement(join(2852..3910,1..970))
                     /label=ENO3-204
                     /note="primary transcript ENST00000519266
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     2947..2966
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: GTTGGGAGAGATGAGGTTGG; added:
                     2024-02-22"
     misc_feature    3034..3133
                     /label=Donor Template SNV -> Rev
                     /note="color: #00ccff"
     primer_bind     3034..3133
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     CTAGCCTCCTTTCTCTTTCCTCCTCCTCCAGCGCTAGTCCTGCTGATGGGCAAAGAAGG
                     TGTCCACGGTGGTTTGATCAACAAGAAATGTTATGAAATGG; added: 
                     2024-02-22"
     misc_feature    3082..3101
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     primer_bind     3082..3101
                     /label=gRNA Protospacer
                     /note="color: black; sequence: GGCAAAGAAGTTGTCCACGG; added:
                     2024-02-22"
     misc_feature    3092
                     /label=SNV
                     /note="SNV = T 
                     REV = G"
                     /note="color: #ff6600"
     misc_feature    3102..3104
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(3397..3421)
                     /label=PCR Reverse
                     /note="color: black; sequence: CACACAGCACCTTGTTAGTAGAATC; 
                     added: 2024-02-22"
     prim_transcript complement(join(6658..3910,1..810))
                     /label=ENO3-209
                     /note="primary transcript ENST00000520221
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     gene            complement(join(8569..3910,1..970))
                     /label=ENO3
                     /note="gene ENSG00000108515
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 cgggcgcgct cccgtgcagc cggctccggc cccgaccgcc ccatgcactc ccggccccgg
       61 cgcaggcgca ggcgcgggca cacgcgccgc cgcccgccgg tccttccctt cggcggaggt
      121 gggggaagga ggagtcatcc cgtttaaccc tgggctcccc gaactctcct taatttgcta
      181 aatttgcagc ttgctaattc ctcctgcttt ctccttcctt ccttcttctg gctcactccc
      241 tgccccgata ccaaagtctg gtttatattc agtgcaaatt ggagcaaacc ctacccttca
      301 cctctctccc gccacccccc atccttctgc attgctttcc atcgaactct gcaaattttg
      361 caataggggg agggattttt aaaattgcat ttgcaaagtt cggtgtctgg gctggcgagt
      421 gggggaggga gggaatgggg agtaggcccc gcccctaccg tcctttgcaa ataaaaatct
      481 agcggggcgg ggggggggag gagcaggaag tggcggtgcg agggctgctg cacagcgagc
      541 ggagccgcgg tccggacggc agcgcgtgcc ccgagctctc cgcctccccc cgcccgccag
      601 ccgaggcagc tcgagcccag tccgcggccc cagcagcagc gccgagagca gccccagtag
      661 cagcgccatg gccgggtgga acgcctacat cgacaacctc atggcggacg ggacctgtca
      721 ggacgcggcc atcgtgggct acaaggactc gccctccgtc tgggccgccg tccccgggaa
      781 aacgttcgtc aacatcacgg tactgcgagg cctgcgcggt ccggggcact gctccggttt
      841 ggaccctgag ggagggactt gggtgggggc gggcgcggtc tagggcgcgc agcgagaggc
      901 cctgactggg tgcttgccct ggaagcggcg cgaatggcgg ctgcacgtga gtgggtccct
      961 cagttgcccg taggtcctat gccccaggcg gcggcgccca tcccgcccgc taccccgctt
     1021 tccgcgacgg ggcgttacat ccggggcaca gggcggggag gggcgcggcg cccggtgcgg
     1081 cggcccactt ccgcctctcc cagggcgggg cggggacgcg cctcagtttt tactggggat
     1141 tgggattgag aggggacccg gggatccttg gacccccagc tccccgtccg gctgccagcc
     1201 cgcgtccctc taccattgtt tctcctggtg cggaagggag ccccaggcgc gtggtcttgg
     1261 tcccttgccc accctctgtg ggcttctggc ccctcctaac tccgttagag agggcgaggc
     1321 caccacacgc ccccccagct cagcacgttg gattcaacat tcctgcgagg ccaatcccgg
     1381 aagtcccctc gccctccctt cccgccatcc ggcggggcct ggagcagggg aactttgtga
     1441 gaagttctga gacagcataa gcgaaagtat attggtttac taccgctgct cccaccacca
     1501 tctccctgtc cacactgccc ggaagtgggg agggggagcg gaagttcaaa agggggggga
     1561 agggggtttc cgggcgggag gggaccggat gtgggggctt ggggtgaaag ggggaggggg
     1621 gaaaggttcc cggggtacct agacccgaga gctggcctgc cgggtctggc ccctgtccac
     1681 cttcaaaagt tttccaactt tcccggaaag cccccagggg tctgcttcct catctcagag
     1741 cagagatacc tggagctaaa ctctaggtta cttggagacc caggcgtccg ggccctaaca
     1801 gctcctgcag aacctttgac caaataaggg caaagttgct tctcttgcct cgcccgcccc
     1861 cctcccttcc cctctccgct tctgcttttc ctgaaggaga gttctgagca tccaaaggct
     1921 cctcacttac ctcactgact ccctttattt cctgggttta ctttccccat taacactgac
     1981 atctaaatct ctagaggagt atgtcatccc ttcctccctg gttccatatt ctaagtttca
     2041 gaatcatgat ctttttctgc actaacgtcc cacagtcctc agagtttctc acagttcatt
     2101 gatgtgtact gagtatataa tttatactta gcccccacgg cggggtggtt ctcagaagtg
     2161 gaagacaggt gggtctttgg agaacacggt gggaatcttg gtgcactgac taacttgatg
     2221 ggcgcttggt tcctcttcca gccagctgag gtgggtgtcc tggttggcaa agaccggtca
     2281 agtttttacg tgaatgggct gacacttggg ggccagaaat gttcggtgat ccgggactca
     2341 ctgctgcagg atggggaatt tagcatggat cttcgtacca agagcaccgg tggggccccc
     2401 accttcaatg tcactgtcac caagactgac aagagtgagt tcctgagtct tacccttcct
     2461 ttagctccaa gatctctaat gtaccgcctt ctggtaatct tgagggtgct tttttgtgtg
     2521 attccccaac atgaagggag tcagaccagt attgtgttcc ttgtctgctg gatgctggaa
     2581 cacgggatag tccctccatt ctgtctcagt tcctcatctt gtaattcgaa gttgctgaac
     2641 tgcattacct cttaaattac ttactgcctt gagctcggat tagaaactgt tttctaggag
     2701 tataggtaga ttgttggggt cttgaatcat cgagttcatc gtttgagttc ttttgttaga
     2761 acctggaggt ggcatgcaca tccacagccc tttctagcct gaacacatga atcaacagtc
     2821 tatgagcctt tgacccctaa gtttttagtc tgttcctctc cagcgtttaa gcttgatttt
     2881 cctcttttga aaagccttat tgtttcaggg tgactgacag cctgcctgtg tgtggtgggg
     2941 gggcgggttg ggagagatga ggttgggtta cagcccccag ggctggacag ctgctgaaca
     3001 gctggcaggg ggtggaattg tgacacctgg atcctagcct cctttctctt tcctcctcct
     3061 ccagcgctag tcctgctgat gggcaaagaa ggtgtccacg gtggtttgat caacaagaaa
     3121 tgttatgaaa tggcctccca ccttcggcgt tcccagtact gacctcgtct gtcccttccc
     3181 cttcaccgct ccccacagct ttgcacccct ttcctcccca tacacacaca aaccatttta
     3241 ttttttgggc cattacccca taccccttat tgctgccaaa accacatggg ctgggggcca
     3301 gggctggatg gacagacacc tccccctacc catatccctc ccgtgtgtgg ttggaaaact
     3361 tttgtttttt ggggtttttt ttttctgaat aaaaaagatt ctactaacaa ggtgctgtgt
     3421 gatttgagct tgggtgtcag aatgggggat aggcactgtg acttgcctcc tttatcgtgg
     3481 cttgggagca gggaggttga ggtactacaa ggtgcaggaa tttgagcttc tggccaggtg
     3541 gccctgcccc tccacagtgg catttccagg cctaagtcct cagtgtgctg gtcaaattgg
     3601 tggatgatgg aatagagcca tctggaccag agaatgggtg caggcctgca ggcaaggcag
     3661 ggctagttga gtcatccttg tgggtggggc tcccccttct gagtcatagc aaatgccggc
     3721 tggggctgtc cccatgacta taggttagtg tctctcagca ttcccttgct ccttatggct
     3781 aaaggggtga ttccaccaaa gggttaagtt cacctgtgtg agaatgatct tacccttcct
     3841 tagtccacaa agaaacaatt ttattagccc aagaagtgaa gacccagtcc ctctgcttgc
     3901 cccagctcca
//
