LOCUS       CZK2J00160_SOD1_        9266 bp DNA     linear   UNA 07-NOV-2023
DEFINITION  superoxide dismutase 1 [Source:HGNC Symbol;Acc:HGNC:11179].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000142168
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..9266
                     /label=SOD1
                     /note="gene ENSG00000142168
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..9266
                     /label=SOD1-202
                     /note="primary transcript ENST00000389995"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 28..9266
                     /label=SOD1-201
                     /note="primary transcript ENST00000270142"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 28..7676
                     /label=SOD1-204
                     /note="primary transcript ENST00000476106
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 44..9266
                     /label=SOD1-203
                     /note="primary transcript ENST00000470944
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(105..119,4125..4221,6784..6853,7593..7710,8806..8913)
                     /codon_start=1
                     /label=SOD1-202
                     /note="coding sequence ENSP00000374645"
                     /note="This feature has 5 segments:
                        1:  105 ..  119 / #993366 / ENSE00001723323
                        2: 4125 .. 4221 / #993366 / ENSE00003492976
                        3: 6784 .. 6853 / #993366 / ENSE00003624439
                        4: 7593 .. 7710 / #993366 / ENSE00003555033
                        5: 8806 .. 8913 / #993366 / ENSE00003902052"
                     /translation="MATKAESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPH
                     FNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEK
                     ADDLGKGGNEESTKTGNAGSRLACGVIGIAQ"
     CDS             join(105..176,4125..4221,6784..6853,7593..7710,8806..8913)
                     /codon_start=1
                     /label=SOD1-201
                     /note="coding sequence ENSP00000270142"
                     /note="This feature has 5 segments:
                        1:  105 ..  176 / #993366 / ENSE00001507447
                        2: 4125 .. 4221 / #993366 / ENSE00003492976
                        3: 6784 .. 6853 / #993366 / ENSE00003624439
                        4: 7593 .. 7710 / #993366 / ENSE00003555033
                        5: 8806 .. 8913 / #993366 / ENSE00003902052"
                     /translation="MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHG
                     FHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSV
                     ISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ"
     gene            4641..4817
                     /note="gene ENSG00000238390
                     snoRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 4641..4817
                     /note="primary transcript ENST00000458922
                     snoRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     gene            complement(7063..7582)
                     /note="gene ENSG00000273271
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(7063..7582)
                     /note="primary transcript ENST00000609934
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     7098..7122
                     /label=PCR Forward
                     /note="color: black; sequence: tacaagtgccaaaggggaactaata; 
                     added: 2023-11-07"
     primer_bind     7408..7427
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: ccccatctttcttcccagag; added:
                     2023-11-07"
     misc_feature    7599..7698
                     /label=Donor Template WT -> SNV
                     /note="color: #00ccff"
     primer_bind     complement(7599..7698)
                     /label=Donor Template WT -> SNV
                     /note="color: black; sequence: 
                     GCCAATGATGCAATGGTCTCCTGAGAGTGAGATCACAGAATCTTCAATAGACACATCGG
                     CCACACCATCTTTGGCAGCAGTCACATTGCCCAAGTCTCCA; added: 
                     2023-11-07"
     misc_feature    7617..7636
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     7617..7636
                     /label=gRNA Protospacer
                     /note="color: black; sequence: GACTGCTGACAAAGATGGTG; added:
                     2023-11-07"
     misc_feature    7625
                     /label=SNV
                     /note="WT = A
                     SNV = C"
                     /note="color: #ff6600"
     misc_feature    7637..7639
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(8187..8211)
                     /label=PCR Reverse
                     /note="color: black; sequence: aggtaaaagagagcctgtctacttg; 
                     added: 2023-11-07"
ORIGIN
        1 gtagtcgcgg agacggggtg ctggtttgcg tcgtagtctc ctgcagcgtc tggggtttcc
       61 gttgcagtcc tcggaaccag gacctcggcg tggcctagcg agttatggcg acgaaggccg
      121 tgtgcgtgct gaagggcgac ggcccagtgc agggcatcat caatttcgag cagaaggcaa
      181 gggctgggac ggaggcttgt ttgcgaggcc gctcccaccc gctcgtcccc ccgcgcacct
      241 ttgctaggag cgggtcgccc gccaggcctc ggggccgccc tggtccagcg cccggtcccg
      301 gcccgtgccg cccggtcggt gccttcgccc ccagcggtgc ggtgcccaag tgctgagtca
      361 ccgggcgggc ccgggcgcgg ggcgtgggac cgaggccgcc gcggggctgg gcctgcgcgt
      421 ggcgggagcg cggggaggga ttgccgcggg ccggggaggg gcgggggcgg gcgtgctgcc
      481 ctctgtggtc cttgggccgc cgccgcgggt ctgtcgtggt gcctggagcg gctgtgctcg
      541 tcccttgctt ggccgtgttc tcgttcctga gggtcccgcg gacaccgagt ggcgcagtgc
      601 caggcccagc ccggggatgg cgactgcgcc tgggcccgcc tggtgtcttc gcatccctct
      661 ccgctttccg gcttcagcgc tctaggtcag ggagtcttcg cttttgtaca gctctaaggc
      721 taggaatggt ttttatattt ttaaaaggct ttggaaaaca aaaatacgca acagagaccg
      781 tttgtgtgac actttgcagg gaagtttgct ggcctctgtt ctaggtcatg attgggctgc
      841 aagggcagag aaggtagcct tgaacagagg tccttttcct cctcctaagc tccgggagcc
      901 agaggtttaa ctgacccttt tggggatttt tgagggcagt gatcttaact ttgggtgcac
      961 agttagctta tttgaagatc ttactaaaaa tacaccagag cccaacctcc gaccaattac
     1021 atcaaaacct gtcctagtgc agggtgagta ttgctgtttt ttgaaagttt ccaaaagtga
     1081 ttttgatgtg cacctacgat tgagaactgt cgtttgagga cagtgggtgg agtttcgtat
     1141 ttggaaatta gaagacctgg agtttccatt acaccgaatt ggcacttaat aactgttgtc
     1201 ggagcatttc ttaagccaca ttttcgtaaa gtggctttaa aattgctctg ccagtaggca
     1261 ggttgctaag atggtcagag acaaacttct gaacgactct tgtaaaatat acagaaatat
     1321 tttcagaact tttatcagta aaattacaaa acgtgttgca aggaaggtgc ttgtgataac
     1381 actgtcccca gaaccttagt gaagttacca actggtggaa aattttctct tgcactcggc
     1441 ttaaaaatca tgagggaata tttactatac gaatgagatt cagtctttaa aggggtttac
     1501 agaaacgtga gaggacagga acagttagtc tgtgtaaatg tctgaaatat atgtgaggga
     1561 gataatgagt ttagcctttt tctttaatag gtctccagat tttctggaaa aggttctttg
     1621 gcatttgact ccattttgct gtttcatttg tcagacttct ttttgtccct ctttacttct
     1681 ccccacataa ttcaccagta ctagtgtttt gtttttcaga ccaagtctcg ctctgtcgcc
     1741 caggctggag tgcagtggcg cgatctcagc tcactgcaac ctccgcctcc caggttcaag
     1801 caattctcct gcctcagcct cccgggtagc tgggactaca ggcgcgcgcc gccacgcctg
     1861 gctaattttt tatattttag tagagacggc gtttcaccat gttggccagg atggtctcga
     1921 tctgttgacg tcgtgatcca cccgcctcgg cctcccaaag tgctgggatt acaggcgtga
     1981 gccaccccgc ccggccacca gtgctattct taagacgcct ctgaggaatc ccttctccct
     2041 ggccattgag aatccatgca tgaacccagg ttttccacct tccctgagca gcttgcatag
     2101 ttccttcttt taagcgcctg acttcgtttt gtttggtgcc cgttgtacct gagaatgagc
     2161 cttggatagt ggagcattcc agctttccag atatgcagag ataatacatt ggctatcagc
     2221 tacttggctt ggcctattcc gtgtttaaaa tcttggactc tttgctagtt tttacagatc
     2281 agaatttttc acgtattaat ccagttttcc tagcttctct tgaagaattt ttggagatct
     2341 cttcatactg agccttcatt agcccaggac agtactgctg tagcagttca tatatttttt
     2401 cgcttcccag gcctgtgtta ttcacttaag ttcatagcct ggtccctgca gggttgtacc
     2461 cgagcacagc tacttagatg tcctgaatgt attaccggtt aaatggaggt ttcaaagaac
     2521 ctgctgtttt tggccctgtg ctcttgataa cagagtgttt gagggacaac tttcacattt
     2581 gagtttttcc aaaattaaag gttgtagaag agtcacagta tctattgtca aaaagaaaag
     2641 aatttaaaaa ggcagcaatt gccaggatac ttcatttgag caatgatatt ttccagtgga
     2701 aagtcacatc ttaagggtta atgcccctta actgttggcc gtatttgaaa acaaaccaag
     2761 ctaaaaacaa gagacactga catgttgtat gacggtgtgg tgtggatgtt gtgtttattt
     2821 tagtcctgag atctagttgt aacttccttg atttctgtat gtagccacgg agcaccatta
     2881 cctgtcacca ttacctgaat ggctatactg cttgctttca ttttggtaga gtggaaaggt
     2941 tacctaggtt tcagtgcttg aaaagatttc agaaagcagt agtacgtctg gttagactag
     3001 aatcagtcct ctcctggggg cagtggaata taatattttc tgactgctaa ttaaaaatac
     3061 ctgtgatagc cgggcgtggt ggcttacgcc tgtaatccca gcactttggg aggccgagac
     3121 gggtggatca cgaggtcagc agatggagac catcctggct aacacggtga aaccccgtct
     3181 ctactaaaaa tgcaaaaaaa ttagccgggt gtggtggtgg gcgcctgtag tcccagctac
     3241 tcaggaggct gaggcaggag aatggcatga acctgggagg cggagcttgc agtgagccga
     3301 gatcatgtca ctgcactcca gcctgggcga cagagcgaga ctcgtctcaa aaaaaaaaag
     3361 aaaaaaactt atgatggaca cttaaaaaca ctcactgagt ggggagtgga gagcaggggt
     3421 cccagggtag cctgttggac atttccaggg cgactttttc tttttttttt tttaaagtca
     3481 agtgagtatg ccatatggaa aagggtgtgc gtggagaaaa agcaaggggc tccagagtgt
     3541 aggatgagac atacaccttt tgggttaaaa aggctgaggc aggagaatgg cgtgaacccg
     3601 ggaggcggag cttgcagtga gctgagatca tgccactgca ctccagcctg ggcgacagag
     3661 cgagactctt gtctcaaaat aaaaaacgtt tacatgtaca tgtatattca acatgtacaa
     3721 atataaccta ttcaaaagta tttactacat aaataggtac ttacattacc tatttactgt
     3781 aatagtcaaa gcctatgaag tatctaacac tgatgtgtag gtactcactt tgcttgccac
     3841 tctattaggt gctttttatg ttatttaatc atgaagcctg gccacagggt gcttgtgcat
     3901 tgagtgtggg aacaagatta ccatctccct tttgaggaca caggcctaga gcagttaagc
     3961 agcttgctgg aggttcactg gctagaaagt ggtcagcctg ggatttggac acagattttt
     4021 ccactcccaa gtctggctgc tttttacttc actgtgaggg gtaaaggtaa atcagctgtt
     4081 ttctttgttc agaaactctc tccaactttg cacttttctt aaaggaaagt aatggaccag
     4141 tgaaggtgtg gggaagcatt aaaggactga ctgaaggcct gcatggattc catgttcatg
     4201 agtttggaga taatacagca ggtgggtgtt gtgctgtgct ggtgacccat acttgttcac
     4261 cctagttaga taaacagtag agtagcccct aaacgttaaa acccctcaac ttgtttttgt
     4321 ttttgagaaa gggtcttgct ctgtcgctca ggctggagtg cagtggcgct gtgcgatcat
     4381 ggctgacctt agccttgacc tcccaggctc cattgatcct catgccttgg cccgtagctg
     4441 ggactacagg tacacaccac cacgcctggc taatttttgt atttttttct agaggtgggg
     4501 tttcatcatg ttgcccaggc tggtcttgaa ctgctgggct caagtggtct atcctcctcg
     4561 acctcccaaa gtgctgggat tacatgtgtg agccactgtg cctgggaaaa ccctcaactt
     4621 ttcttttaaa aaagaggtca actttattgt atataagcac tgtgctaaaa ttgcaggaac
     4681 tgggaccata tcctgatttt tgtaataatg ccagcagagt acacacaaga aaagtaactg
     4741 cactagattg tgaagactgg ggtggacctg cttctgaagg tccagtgccc tttgtcttaa
     4801 gatttggtgt agtgtgtctt tagaaaccaa aaaaagagaa gaagatcaac cttaagatta
     4861 gccacaaaac tgggctttga tacctaggtg tggaaaagaa agggaaagag ttgatgtttt
     4921 gtcttacagc atcattgtag aagagggtgt ttttttgttt gtttgttttt tgagacggag
     4981 tcttactctg tggcccaggc tggagtgcag tggcgcgatc tcggctcact gcaagctccg
     5041 cctcccgggt tcatgccatt ctcctgcctc agccccctga gtagctggga ctacaggtgc
     5101 ccgccacccc gcctggctaa ttttttgtat ttttagtaga gacggggttt cactgtgtta
     5161 gccaagatgg tctctctcct gacctcgtga tccgcctgtc tcagcctccc aaagtgctgg
     5221 gattacaggc atgagccacc gcacccagcc agaagagggt gttttttaaa gaaggcaaat
     5281 aggaaataaa aacttgggct cttaactttt gtaatgatcc caggtgtttg agctgggggt
     5341 tgagggtggg tgcctcgagc aaaggggctg catttatttg cataatgcca tgtaagagta
     5401 gctctacacc ccaaacacag gcttcttagt gggaccaaag tatgatacaa actgaagatg
     5461 gaatgcagag gattattggt actttggaat atgcttaaaa aaaatttttt taaagtattt
     5521 ttaaaaaatc aggcaacccc tgaaccagag taggttcaga gaaactgcca aattttattt
     5581 tcttaatttg ggattggaag caagttaaca gaagtttatg agttaagttg catttagtga
     5641 tcttttgcca tatttgagta ataatctgat ttttttgttt atagatttct tcttaaatta
     5701 actttattca tcttgctaat ttagtttcaa atagtgattt gtaatgatca gatttgatcc
     5761 atttctgtaa ttgctgaaat tcccccgagt tgctttttgg ctttaccgcc tctggtctgg
     5821 gaggtgattg ctctgctgct tcctgtaact tgcctgcctt tctccctgtg tgggactcct
     5881 gcgggtgaga gcgtggctga agacagccgt gttatgaaag ggcctcctgt gctgtcgagg
     5941 ttgtgctctg tgaatgtcat cccctggtgc acagcagcac cttctacaca ggatacagtt
     6001 ggaatgccgc cccctcgagt tgtgtaaggc agcagccttg gcccttgcac ataagatgct
     6061 gttgaatatt ctgcctgcac caagtaaagg gcacagatag aactgcttgg catatgttgc
     6121 tggggagatg agttttttgt aaagtatact acgttcttaa gaatttggat cataaccatg
     6181 ggattttaat aatagaaaaa ctgttgaaga tcagtctggt cccttatttt tacagtgaag
     6241 aagccaaagc ccagagaagg gtgttaactt tacaagtgtc agacagtagt tagaacttgg
     6301 tggggttttt tttttttttt ttttgagatg gagtcttgct ctgttgccca ggctggagtg
     6361 cagtggtgcg atctcagctc actgcaacct ctgcctccca ggttcaagcg attctcctgc
     6421 ctcagcctac taagtagctg ggactatagg tgcgcaccac cacgcctagc taatttttgt
     6481 attttttcag tagagacagg gttttgctat gctggccagg ctggtctcaa actcctgacc
     6541 tcagatgatc cagccacctc agcttcccaa agtgctgggg ttccaggtgt tagccaccat
     6601 gcctggccat agacttgttt ctgttccctt ctcactgtgg ctgtaccaag gtgttgctta
     6661 tcccagaagt cgtgatgcag gtcagcactt tctccatggg aagttttagc agtgtttctt
     6721 tttagaatgt atttgggaac tttaattcat aatttagctt ttttttcttc ttcttataaa
     6781 taggctgtac cagtgcaggt cctcacttta atcctctatc cagaaaacac ggtgggccaa
     6841 aggatgaaga gaggtaacaa gatgcttaac tcttgtaata atggcgatag ctttctggag
     6901 ttcatatggt atactacttg taaatatgtg ctaagataat tccgtgtttc ccccaccttt
     6961 gcttttgaac ttgctgactc atctaaaccc ctgctcccaa atgctggaat gcttttactt
     7021 cctgggctta aaggaattga caaatgggga cacttaaaac gatttggttt tgtagcattt
     7081 attgaatata gaactaatac aagtgccaaa ggggaactaa tacaggaaat gtcatgaaca
     7141 gtactgtcaa ccactagcaa aatcaatcat cattgtgaaa cataggaagc ttctgtagat
     7201 aaaaaaaaaa attgatactg aaaactagtc gagactccat ttatatgtgt atgttttctg
     7261 aaagcctttc agaaaaatat taaatttaag gacaagattt ttatatcaga ggccttggga
     7321 catagctttg ttagctatgc cagtaattaa caggcataac tcagtaactg agagtttacc
     7381 ctttggtact tctgaaatca ggtgcagccc catctttctt cccagagcat tagtgtgtag
     7441 acgtgaagcc ttgtttgaag agctgtattt agaatgccta gctacttgtt tgcaaatttg
     7501 tgtctactca gtcaagtttt aatttagctc atgaactacc ttgatgttta gtggcatcag
     7561 ccctaatcca tctgatgctt tttcattatt aggcatgttg gagacttggg caatgtgact
     7621 gctgacaaag atggtgtggc cgatgtgtct attgaagatt ctgtgatctc actctcagga
     7681 gaccattgca tcattggccg cacactggtg gtaagttttc ataaaaggat atgcataaaa
     7741 cttcttctaa catacagtca tgtatctttt cactttgatt gttagtcgcg gtttctaaag
     7801 atccagataa actgtacttg cagttcaaat taggaaaagc aattttattg gacaattacg
     7861 gtgaaaatga attattttat ctaggtcagt taagaacact gttctgctaa gatgcagtaa
     7921 aaagcaggtt acatttgacc atattagatc tgagtttgga aaacagaagt agtctttagt
     7981 tttaaaatgg ccagattttc ttgccaggat tgggtttctc acttgttaaa cagaacattt
     8041 tgttaagttt aaaacctggg atggacttaa gtattcatgt tcattcatgt tcattcagga
     8101 ctgcaggtta tcatgacttg tttaacttgt gggaagctgt tgtcccaagt tatcctgggg
     8161 aactgcatct ggttcttgca aaacaccaag tagacaggct ctcttttacc tccccttgag
     8221 ggcattaaca ttcagtagtc acttccattc agttaaccct ttatttttat ggtttttctt
     8281 gagccatagt tgtaaagcag aaaaatcatt tataaaggtt tgttgaacaa aattcaaaat
     8341 actgttgctt aaagtattaa gattttttag gattatacct tacttatagg cccgtcattc
     8401 atttggcatg aaattttgag ttttattcac tttcactttc ctttttttcc aaagcaatta
     8461 aaaaaactgc caaagtaaga gtgactgcgg aactaaggtt actgtaactt accatggagg
     8521 attaagggta gcgtgtggtg gtctacaaca tagttatttg ggttttagta tttcatttag
     8581 acagcaacac ttacctaatg tttaaaggta atgtctttgc aacaccaaga aaaagctttg
     8641 agtagtagtt tctactttta aactactaaa tattagtata tctctctact aggattaatg
     8701 ttatttttct aatattatga ggttcttaaa catcttttgg gtattgttgg gaggaggtag
     8761 tgattacttg acagcccaaa gttatcttct taaaattttt tacaggtcca tgaaaaagca
     8821 gatgacttgg gcaaaggtgg aaatgaagaa agtacaaaga caggaaacgc tggaagtcgt
     8881 ttggcttgtg gtgtaattgg gatcgcccaa taaacattcc cttggatgta gtctgaggcc
     8941 ccttaactca tctgttatcc tgctagctgt agaaatgtat cctgataaac attaaacact
     9001 gtaatcttaa aagtgtaatt gtgtgacttt ttcagagttg ctttaaagta cctgtagtga
     9061 gaaactgatt tatgatcact tggaagattt gtatagtttt ataaaactca gttaaaatgt
     9121 ctgtttcaat gacctgtatt ttgccagact taaatcacag atgggtatta aacttgtcag
     9181 aatttctttg tcattcaagc ctgtgaataa aaaccctgta tggcacttat tatgaggcta
     9241 ttaaaagaat ccaaattcaa actaaa
//
