LOCUS       CZK2J00186_PLAAT        9655 bp DNA     linear   UNA 08-NOV-2023
DEFINITION  phospholipase A and acyltransferase 4 [Source:HGNC 
            Symbol;Acc:HGNC:9869].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000133321
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..9655
                     /label=PLAAT4
                     /note="gene ENSG00000133321
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..9651
                     /label=PLAAT4-201
                     /note="primary transcript ENST00000255688"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 2..9655
                     /label=PLAAT4-204
                     /note="primary transcript ENST00000537871
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 9..8580
                     /label=PLAAT4-203
                     /note="primary transcript ENST00000439013"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 38..9518
                     /label=PLAAT4-205
                     /note="primary transcript ENST00000544107
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(62..70,2709..2817,7814..8082,9342..9449)
                     /codon_start=1
                     /label=PLAAT4-201
                     /note="coding sequence ENSP00000255688"
                     /note="This feature has 4 segments:
                        1:   62 ..   70 / #993366 / ENSE00002295822
                        2: 2709 .. 2817 / #993366 / ENSE00000908980
                        3: 7814 .. 8082 / #993366 / ENSE00003474706
                        4: 9342 .. 9449 / #993366 / ENSE00003552581"
                     /translation="MASPHQEPKPGDLIEIFRLGYEHWALYIGDGYVIHLAPPSEYPGA
                     GSSSVFSVLSNSAEVKRERLEDVVGGCCYRVNNSLDHEYQPRPVEVIISSAKEMVGQKM
                     KYSIVSRNCEHFVTQLRYGKSRCKQVEKAKVEVGVATALGILVVAGCSFAIRRYQKKAT
                     A"
     CDS             join(62..70,2709..2817,7814..8166)
                     /codon_start=1
                     /label=PLAAT4-203
                     /note="coding sequence ENSP00000402943"
                     /note="This feature has 3 segments:
                        1:   62 ..   70 / #993366 / ENSE00002278599
                        2: 2709 .. 2817 / #993366 / ENSE00000908980
                        3: 7814 .. 8166 / #993366 / ENSE00002271575"
                     /translation="MASPHQEPKPGDLIEIFRLGYEHWALYIGDGYVIHLAPPSEYPGA
                     GSSSVFSVLSNSAEVKRERLEDVVGGCCYRVNNSLDHEYQPRPVEVIISSAKEMVGQKM
                     KYSIVSRNCEHFVTQLRYGKSRCKQVRTSLDNPSPSAWGWTHGAVQPGDHCE"
     prim_transcript 1589..9650
                     /label=PLAAT4-202
                     /note="primary transcript ENST00000354445"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(1589..1607,2719..2817,7814..8082,9342..9449)
                     /codon_start=1
                     /label=PLAAT4-202
                     /note="coding sequence ENSP00000346431"
                     /note="This feature has 4 segments:
                        1: 1589 .. 1607 / #993366 / ENSE00002450405
                        2: 2719 .. 2817 / #993366 / ENSE00001405564
                        3: 7814 .. 8082 / #993366 / ENSE00003474706
                        4: 9342 .. 9449 / #993366 / ENSE00001312826"
                     /translation="MGLPEPKPKPGDLIEIFRLGYEHWALYIGDGYVIHLAPPSEYPGA
                     GSSSVFSVLSNSAEVKRERLEDVVGGCCYRVNNSLDHEYQPRPVEVIISSAKEMVGQKM
                     KYSIVSRNCEHFVTQLRYGKSRCKQVEKAKVEVGVATALGILVVAGCSFAIRRYQKKAT
                     A"
     primer_bind     2053..2077
                     /label=PCR Forward
                     /note="color: black; sequence: tattgtaggacaagttcccacagag; 
                     added: 2023-10-05"
     primer_bind     2603..2622
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: atgaacatcagcaaggcgtc; added:
                     2023-10-05"
     misc_feature    2718..2817
                     /label=Donor Template WT -> SNV
                     /note="color: #00ccff"
     primer_bind     complement(2718..2817)
                     /label=Donor Template WT -> SNV
                     /note="color: black; sequence: 
                     TTGGAGGAGCCAGATGGATCACGTAGCCATCTCCTATATACAGGGCCCAGTGCTCATAG
                     CCAAGGCGGAAAATCTCAGTCAGGTCTCCAGGTTTGGGCTC; added: 
                     2023-10-05"
     misc_feature    2736..2755
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     2736..2755
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CTGATTGAGATTTTCCGCCT; added:
                     2023-10-05"
     misc_feature    2740
                     /label=SNV
                     /note="WT = T
                     SNV = C"
                     /note="color: #ff6600"
     misc_feature    2756..2758
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(3194..3218)
                     /label=PCR Reverse
                     /note="color: black; sequence: aactcaagtttcccttgcttaactg; 
                     added: 2023-10-05"
ORIGIN
        1 ccttcagcat aaaagctgat ccacaaacaa gaggagcacc agacctcctc ttggcttcga
       61 gatggcttcg gtaagtttcc cagggctttg cattactgac tctacagcag ttgggcagct
      121 ccccctagga aaggcctcag tccaggctcc tggccttggg cacacagctc cagagctcgt
      181 ctttagaagg ctgtcatgga aaactgtatt tgtcttttaa aagtgcatct gcccagctgg
      241 acagggagtc ctctgggcag agtccacctc ctctgcgtaa ctggcacagt gcggggagcc
      301 cgggactccc atgtagtaga gagctgtggg gtgccgggtg tcaaatctac ctcagcctga
      361 ggagaagcca gagtgtgggc aggtggggga ctctgggttg tgggaagcaa gcaaatccca
      421 acatcgtctg cttctgctgt gactggggct cagtcccttc tcggccccaa acgatgcttt
      481 ctctctcctc gttcctctgt ggcatcatcc agccttctaa ggtgcattct aatctcagca
      541 gctttctgaa aactgaaatt tctcctccta tcagcccaga tgtaccgatt ggcagaagct
      601 ctagggagag caggctgagc tcggtggtgt gtgactaaaa aggaacagcg gggctcaggc
      661 tattactaga aacaaaacca ttccgggaag gagtcctgca aattctggag ggcagcccac
      721 tccaacagca ccacctgatc ctggggaagg gctttactgc tgccaaaaca aatcagggct
      781 tttaacaggg agcccaggcc aaagctcata tctcactggc caccagctga ggttgccagt
      841 accttgactg tggttttaga cattcagcat tgcagttaaa agcccctctc tctccccaca
      901 tggaaaccat ctcctgtggt gccctgacat acagaggagg agctgggagg gagtcctggc
      961 cctgctcctt tctcctccct cttccttgtc tatttccttg ccccattcta cgccaggccc
     1021 ttcactgggt cctggaggtg cagggaggga ggagcacaca ggatcctgcc tgcctggagc
     1081 tcacagccca gaggggcaca gggaggcaca gggaattgca gctcaacttg acccctgcag
     1141 agagcagcag aggcccccgg ggacccaggt cctaggagtg gggtggcggg tcagctgagc
     1201 caggctgggg ccatcagggc agcttctcag gtgagggggt ttgagggagg ggaagaggaa
     1261 gagtggctgc agggaagtga tggaggcagg gagaaccaga tgtgcagagc agcagagacc
     1321 agagccaggg cagtagtgag gggctgggat gtcactcagt gggcctgtgg gtgcagagcc
     1381 cagggaggga gaggagggtg aggaagggcc agtcaggaag ggcctgggag ctgcccacag
     1441 ggtgtgtcct gggccctgag gtcagaggac ggtcatggcc agatgtgccc ttgggaaggc
     1501 caccatgggt tctgaggagt tggatatgga gtggcaggga caggaggcag gggctggtca
     1561 gggggttgtg ggggtcacag aggcaggcat ggggctgcct gaaccaagtc agtggatggg
     1621 atattgacaa gcacaccaac aaggagaagc ttccggagtc cacattgatt ggttttagtg
     1681 actggatatt ggcatgagga agagggcaaa ggagagcaga ccccaggtct atgatgggac
     1741 atcctgggat gagggaggag gaggctgtca cccactgagg acagggacag agagggtgaa
     1801 aatggaaaaa tcctccagtc atgaggctgc tatgccctgg ggcaggacat ggagaagtgg
     1861 gaaggaggtg aggaagggga agaaggtggc caagacagga accagggtga ggggtgtgca
     1921 gggaagaacc agctcggcct ccttccagct gagtgttctc aggaaagccc tgccacctct
     1981 ctgatcctca gtttccccat ctgtgcaatg ggatgatcat gccttcccct ggggctgtgg
     2041 agatggtgca gttattgtag gacaagttcc cacagaggcc tgttctgtga gtgcagagcc
     2101 ccctcagtgc tgggagctgg ggggccccac aagaccccac tcaggtccca gcaggatgct
     2161 gtgagctcct gtctcccagg gtgtcatctc caagagatag gtgtcctgag gctggatggg
     2221 gcaaggtctg aggaggaggt ggctcttggg gccatggaga ggcacagggc aggagtgatg
     2281 aggaactgag ggtcagtctg cagagggcgg agagctcaag actcaccttc ccagccttgc
     2341 tcctggcctc agtgaaggtg ctggttcatg caggctcttg agtcggccta ctgaggagca
     2401 tgtgtacaat gcacacatgc acacacatct gcatcctcag catgcacaca cacagcacac
     2461 acatgtcaca catgcgccca gacggtcctt ctgtctccca tcccacaaga tcattctcca
     2521 ggcttccagc ccccagtgcc cccactgaga agagcctctg aaagtgacag cagggctgag
     2581 gatctcatgg ggctagcagc tgatgaacat cagcaaggcg tctccaggat gagctgcagc
     2641 tcccccagcc agggtctctg ctgttgccta gaaggccggg tactccatct ctggcttttc
     2701 tgttgcagcc acaccaagag cccaaacctg gagacctgat tgagattttc cgccttggct
     2761 atgagcactg ggccctgtat ataggagatg gctacgtgat ccatctggct cctccaagta
     2821 aggactgatg aatatataat tttcaaaata tttgttaaaa ggataatgag agggccgggc
     2881 acggtggctc atgcctgtaa tcccagcact ttgagaggcc gaggtgggca gattacctga
     2941 ggtcaggagt tcaagaccag actgaccaac atggagaaac cccatctcta ctaaaaatac
     3001 aaaattagcc aggtgtggtg gcacacacct gtaatcccag ctactcagga ggctgaggca
     3061 ggagaatcac ttgatccccg gaggcagagg ttgcagtgag ccgagatcgt gccattgcac
     3121 tctagcctgg gcaacaagag caaaacttga tctcaaaaaa taaaaaaata agtcaatgag
     3181 agccactgaa agacagttaa gcaagggaaa cttgagttag gagacacatt cttcttagag
     3241 gatgcttgat gagattgcac acacatatgt cccatgaagt agacacagtg cattccatag
     3301 gtcctggcag aacggaagaa gagaccgttg cctcccgcag cgaagctggg ctgcttcagc
     3361 attgggatct ctccagctca aagtcagtta ccttggcatg cagaatctca tacgacttaa
     3421 accagccagg tttcttaggt cttaaagaag aaagtttctc aaaaggaaca atgtttctcc
     3481 gtgccagatc ctttgcctca tatgttttca cacaacaagg tgtcttgata ttatatactt
     3541 aagattcctt actgggagca attctcacag gtttatacat ttttttaaaa ttctcataca
     3601 aatacacagt aagcaatacc aatgttttat ttaactcatt aataacagaa gcagaaatat
     3661 gacataccca gttaaaagca gaatgaagga aacgtatgta tggatagcca atgaaagggg
     3721 aaaaaacgaa cacagaggcc aggcacagtg gctcactcct ataatcccag cactttggga
     3781 ggccaaggcg gacggatcac ttgaggtcag gagttcaaga cgagcctggc caacatggtg
     3841 aaaccccgtc tctattaaaa atacaaaaat tagctggggg tggtggcagg catctgtaat
     3901 cccagctact caggaggctg aggcaggaga attgcttgaa cctgggagac ggaggttgca
     3961 gtgagccgag atcctgagac agtgccactg cactccagcc taggagacag aggaagacat
     4021 tgtctcaaaa aacaacaaca aaaatgcaga aatgagacaa tgagactgcc aaattaggta
     4081 ggtgcaggca agttcatatc ctctagggca gtgccactac ataacttctc atgttctctt
     4141 ttcaccaagc atttacattg tcactggaca aaaaccattc ccatcactat cagggcaaga
     4201 catttgcatc ttatcagttt tctccaagcc atcatctccc aggcaggatt gtaaaaatgt
     4261 ctgaattgat ggaaaatagt aaatcaagcc cacttaatcc agatgatcct cagggacctt
     4321 gtaagacaaa gcatggcaat gaagacacat ggtccttttt gtgcctaact tgcagtgtgt
     4381 aaagaggctt tttttttttt tttaagacag agtcttgctc tgtcaccggg atggagtaca
     4441 gtggtgcaat ctcagctcac tgcaacctcc gcctcccagt ttcaagcgat tcccctgcat
     4501 cagcctcccg aatagctgag gctacaggcg catgccacca cagccagcta gttttttgta
     4561 ttttagtaga catggggttt caccatgttg gccaggatag tctcgatctc ctgacctcgt
     4621 gatccaccag cctcggcctc ccaaagtgct gggattacag gcatgagcca ccgcgctcag
     4681 ccgtaaagag gctttttaaa gacaattttt ctctctttcc tttttttttt tttttttcct
     4741 ctgagacagt gtcttactct gccatccagg ctggtgtaca gtgatgtgat tacagctcac
     4801 tgcagtcttt tttttttttt tgcataaaca ttctgattta ataaatggta tgaatatata
     4861 ttttgcatac tttacaataa agaaaatttt gaaagaaaat tgatctaaaa ctgtttccct
     4921 ccaatttcca tggtaaagtc aactgcatac agttctttcc atgtataagt tttacatttg
     4981 tattttctac aattaaataa atctaaatgt taaattttag cacatatatc caattatgta
     5041 caaaattaca atgtcaattt ataagtcaag cttacttaaa aatcataaat tacaaaaaac
     5101 acattatgaa taattttgtt ctggaagtat cataccaact catacgatct aggtgaaccc
     5161 aaaagtaaaa aacaatgatt ataaattaca gtagtgtctg taaaaccctg ttataaagga
     5221 ggtattcttt taaaatatgt accttatata taaaagctcc tgactccttt taaggaccaa
     5281 aattattttc agtttaacca ttttcattaa aatgcttatt taaaagtatc gccttataaa
     5341 actgagtata taaaatacaa tgcagccctt gataactaag aatctttata aatgctaatt
     5401 atttagtgac ctgtttatca aactatttct gtggatgggg tgattttttt tcttcctcta
     5461 aattaagtgg ccctagacta atatatttat atagttattc tgttttcttc cgtactgtca
     5521 tttcacatct ctatcagtga gttcctcagg atttgccttt attttacaac tatgaaagtc
     5581 ttcaatccaa ttccctgctt ccacacatag gacacagaaa gcctaaactt tccttttttc
     5641 tgggctccaa tgggaagcag catttacaac gtgtctggct tggtaggagg aagattgtca
     5701 attgaggtat catgtgtgtt cacctgccta tcttgcccta tttctttggt tgctgtaaat
     5761 ttcctgcttc tcaaatatct ggatttttgg ctacctattt ggcttccgtc tttggtgttt
     5821 atatctgtct ggttttagca ggtctctctg attcctgact cacagctcct ctccttcact
     5881 aatattcaag tgtgctgctt gcctaactcc tcattccttc tccttgaatt tcactttaca
     5941 attgcactag gttctatcat cgttggccac agattaccac aaaacataat ttcttaaatt
     6001 ctgcacattt ctaatgcttc taatcttggt ttcctacaag atctaacact aagcttgtgt
     6061 tatcctgatt acagtgtatg taaaacaatg cttgtggaaa actaacctag gagaaagctt
     6121 ataggggaaa cctgataatg aattttatta aattaataac ccttataaaa atttcaaatg
     6181 taatactacc tggatttgaa cttcaatact tttcttaagt aaaattctta accacggtag
     6241 aagttatgtt tgttgatgtt cactcatcta taccctccat attctcagca aagttggcat
     6301 aaatctgacc tgaataaata gggttataac attatcaggc ttgtaaaata ctttatcaaa
     6361 tcaatgcatc agttaaagca ggggtcccca accctgggct gtgtgtggcc tgttaggaat
     6421 cgggccacac aataggtgag ctgagaggca ggtgagtgag cattctgcct gagctccgcc
     6481 tcttgtcaga tcagtagtag cattagattc tcataggagt gcaaacccca ttgggaactg
     6541 cacatgcaag ggatcaggct cactgcagtc ttgacctccc cgagttcagg ccaggtgatc
     6601 ctcccacatc agccttctga atagctgaga cgagatgata ggcgtgtgcc accacgcgca
     6661 gctaattttt gtattttttg tagagacagg tctcgccatg ttgtccaggc tggtctcgaa
     6721 ctcctgagct caagtggtcc acctgtttag gcctcccaaa gtgctgggat tacaggcatg
     6781 agccactgcg cccagccaac aggattttct taagagggtt ctaaatgcaa acatcagcct
     6841 tgagattaga aacggggcaa aagaggtaac acacctaatt attgtgtgca ttaggtacta
     6901 tccattttaa taaaaataag ataacacctg gagtcactat ataatggaat tggggctcca
     6961 ttggttgaca gctcttctgt gttcctctct atgtgcctag aactgtgcca gattgtagga
     7021 cagaagacac tttacaaagg attccctcag ggtcagccca gtgcagtgga aggagcctgc
     7081 cctttggggc ttaagagaag cagtttcaca tcctgattct aggacttttc atagcacaga
     7141 aaagtctcat gatgtctctc agcccaatat tggtttttaa attgagatga taatattttc
     7201 cttgaaagtt aaaaaggaga ttaaattagg aagtatgtgt aacagcacag catggtaact
     7261 gaaacagtgg gtactaaacg aattggatct ttttccgaaa cacaagtgta caaagctgaa
     7321 caggtaataa atatgcttta ccatcattgt ttataatgat gaaaattttt ccaataccta
     7381 aatgacggat aacaggggat tggccacaga agtgatgtac atgcatagca tggaatatgg
     7441 cagcagccac tggcaatcat gacattcact gacttgaacg aacattcaca ccttatttta
     7501 ttaaaaaaag agagcaaaat ctattagccc ggcgtggtgg tgggtgcctg tagtcccagc
     7561 tactcgggag ggtgaggcag gagaatggcg tgtacccagg agatggagct tgcagtgagc
     7621 tgagatcgcg tcactgcact ccagcctggg caacagagtg agactccatc aaaataaaaa
     7681 aaaaaagcaa aatctgtgaa tccttcaatc acatttgtga aaggataaac accagtagtt
     7741 ccttagtgga gatttcaagc ccttctctcc ctacttcacc ttcccctgcc agtgagagtg
     7801 cctctgattg caggtgagta ccccggggct ggctcctcca gtgtcttctc agtcctgagc
     7861 aacagtgcag aggtgaaacg ggagcgcctg gaagatgtgg tgggaggctg ttgctatcgg
     7921 gtcaacaaca gcttggacca tgagtaccaa ccacggcccg tggaggtgat catcagttct
     7981 gcgaaggaga tggttggtca gaagatgaag tacagtattg tgagcaggaa ctgtgagcac
     8041 tttgtcaccc agctgagata tggcaagtcc cgctgtaaac aggtaaggac ctctctggat
     8101 aatccatctc cctctgcttg gggctggact cacggggctg tgcagccagg cgatcactgt
     8161 gaatgatgca aatgcagcct cttagagacc tgctggctga gagtcaggac ctctgagctc
     8221 caatgccaga tctgcttcta atccacagag ggtctttgga cagttccctt ccccccaggc
     8281 ctctccatgt gcagaggagg gggtggatgg aggagctctg aggatccttc ccctctaaaa
     8341 cctatcctga aaatggctcc tgaagttctg ggggtcatca ggagaatctg ggccaaccca
     8401 ggacaatgac aggtcctgca accccccaaa gagtactcag gcaagggacg ctcagggtcc
     8461 gtggacctgt gtaagagctc aaggacattt gtgtgtaaga tctgggcaca gcctccatcc
     8521 cttgcccctt cacagtcttt cacatttgtt gccccaaaac actcaactaa taagtggcag
     8581 aaccgggatt tgaaccccag ctgtcccttt ctagagtctc tgcctgtgat gcctactcag
     8641 ggcccctggc ctgcgtgact gaatggttgg gggcccttga tgagagaggg atggtggcag
     8701 gagtagaggc tttgcggagg gaaatccaga gtttggcgtg gcatgtgatg tctgaggtgc
     8761 cccattagag gcccaggtgg agataaggca tggagtttag gctgaagtac agctggagat
     8821 ggaagtttcc aggctgcagt tgggaatagt acacagtgac cccagggcac gcatatggcc
     8881 atggcctccc taagtctccc ctcagcttct gccacagccc tccctgtgca aaggtgtagc
     8941 tcagggagga cccagactgc aaaatagtcc ccagtctggc ccctggagct ctgaccctag
     9001 gaagcgaggc acaaaccttc ttccttgccc caaatcagaa caaatgtcct cacttggaag
     9061 ctctctcaaa aggacctcat gggcccattc tcaggacaag cccagacaag tcgaacacca
     9121 agaagtggtc acagcccaga aaatggagtc cctggggata caaccaacgg cttctctggg
     9181 agaggattct gagccagcca gctgggagag aagagaggag atgccaggcc ttaggggagg
     9241 caggagagag gggagaggga gaggagttcc aggcccttgg gagggagaaa gtgccagcct
     9301 ggcttgccgt gaacgctcaa caaaagctgc ttgctttgca ggtggaaaag gccaaggttg
     9361 aagtcggtgt ggccacggcg cttggaatcc tggttgttgc tggatgctct tttgcgatta
     9421 ggagatacca aaaaaaagcg acagcctgaa gcagccacaa aatcctgtgt tagaagcagc
     9481 tgtgggggtc ccagtggaga tgagcctccc ccatgcctcc agcagcctga ccctcgtgcc
     9541 ctgtctcagg cgttctctag atcctttcct ctgtttccct ctctcgctgg caaaagtatg
     9601 atctaattga aacaagactg aaggatcaat aaacagccat ctgccccttc agaaa
//
