LOCUS       CZK2J00153_EIF2B       13361 bp DNA     linear   UNA 08-NOV-2023
DEFINITION  eukaryotic translation initiation factor 2B subunit alpha 
            [Source:HGNC Symbol;Acc:HGNC:3257].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 13361)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000111361
FEATURES             Location/Qualifiers
     source          1..13361
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..13361
                     /label=EIF2B1
                     /note="gene ENSG00000111361
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..7800
                     /label=EIF2B1-204
                     /note="primary transcript ENST00000537073"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 79..3720
                     /label=EIF2B1-202
                     /note="primary transcript ENST00000452159
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 81..13361
                     /label=EIF2B1-201
                     /note="primary transcript ENST00000424014"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 110..3533
                     /label=EIF2B1-206
                     /note="primary transcript ENST00000543940
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 163..11131
                     /label=EIF2B1-203
                     /note="primary transcript ENST00000534960"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(163..222,1321..1422,3234..3370,3482..3598,8905..8946)
                     /codon_start=1
                     /label=EIF2B1-203
                     /note="coding sequence ENSP00000443172"
                     /note="This feature has 5 segments:
                        1:  163 ..  222 / #993366 / ENSE00002208761
                        2: 1321 .. 1422 / #993366 / ENSE00003600970
                        3: 3234 .. 3370 / #993366 / ENSE00003479225
                        4: 3482 .. 3598 / #993366 / ENSE00000757306
                        5: 8905 .. 8946 / #993366 / ENSE00003567322"
                     /translation="SAGGSGPLGLRSRRTPWTTRS*LNTLSLR*KKILTWPQQWLPSGR
                     CWSS*REIKGRQSRV*GRISPVP*KPCVVWTPLWQCPLAGSSSSASSVLPPWNTPITPN
                     VKRS*LSGENFFSGEYHCQETKLQICAILSSKMERYIMEKADLVIVGA"
     CDS             join(210..222,1321..1422,3234..3370,3482..3598,6611..6723,
                     7274..7342,8905..8980,11006..11131,11847..12011)
                     /codon_start=1
                     /label=EIF2B1-201
                     /note="coding sequence ENSP00000416250"
                     /note="This feature has 9 segments:
                        1:   210 ..   222 / #993366 / ENSE00003894441
                        2:  1321 ..  1422 / #993366 / ENSE00003600970
                        3:  3234 ..  3370 / #993366 / ENSE00003479225
                        4:  3482 ..  3598 / #993366 / ENSE00000757306
                        5:  6611 ..  6723 / #993366 / ENSE00001504355
                        6:  7274 ..  7342 / #993366 / ENSE00001504354
                        7:  8905 ..  8980 / #993366 / ENSE00003593306
                        8: 11006 .. 11131 / #993366 / ENSE00003675282
                        9: 11847 .. 12011 / #993366 / ENSE00002206351"
                     /translation="MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGL
                     RANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRI
                     SLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKK
                     MAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQ
                     NKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLI
                     TLLFTDLGVLTPSAVSDELIKLYL"
     CDS             join(210..222,1321..1422,3234..3370,3482..3598,6611..6910)
                     /codon_start=1
                     /label=EIF2B1-204
                     /note="coding sequence ENSP00000444183"
                     /note="This feature has 5 segments:
                        1:  210 ..  222 / #993366 / ENSE00002203722
                        2: 1321 .. 1422 / #993366 / ENSE00003600970
                        3: 3234 .. 3370 / #993366 / ENSE00003479225
                        4: 3482 .. 3598 / #993366 / ENSE00000757306
                        5: 6611 .. 6910 / #993366 / ENSE00001664728"
                     /translation="MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGL
                     RANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRI
                     SLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGQV
                     PFCSVMCPAIILQSKLRITVQQDQNQNVPPACQQSALPFIVPFPAFGRKITEFAAGRSI
                     "
     prim_transcript 1320..12029
                     /label=EIF2B1-205
                     /note="primary transcript ENST00000539951"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(1347..1422,3234..3370,3482..3598,6611..6723,7274..7342,
                     11006..11102)
                     /codon_start=1
                     /label=EIF2B1-205
                     /note="coding sequence ENSP00000438060"
                     /note="This feature has 6 segments:
                        1:  1347 ..  1422 / #993366 / ENSE00002270960
                        2:  3234 ..  3370 / #993366 / ENSE00003479225
                        3:  3482 ..  3598 / #993366 / ENSE00000757306
                        4:  6611 ..  6723 / #993366 / ENSE00001504355
                        5:  7274 ..  7342 / #993366 / ENSE00001504354
                        6: 11006 .. 11102 / #993366 / ENSE00003503213"
                     /translation="MKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCG
                     VDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHT
                     FIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVT
                     VVLDAAVGLEPTRWLCVPKHRTNLSMWLQKVSSLSGSFH"
     primer_bind     6926..6950
                     /label=PCR Forward
                     /note="color: black; sequence: TAAGTAGTTAACTCCGTGGAAGAGC; 
                     added: 2023-09-28"
     misc_feature    7303..7402
                     /label=Donor Template WT -> SNV
                     /note="color: #00ccff"
     primer_bind     complement(7303..7402)
                     /label=Donor Template WT -> SNV
                     /note="color: black; sequence: 
                     TTGCTGTAAGCCAGCATGGGGTGGGGGAGGTGATTATGGCTGGGGAAGATGGGCACTCA
                     CCCGAAAGCAGCATCTAGCACCACAGTGACAGGGACGTTGA; added: 
                     2023-09-28"
     misc_feature    7321..7340
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     7321..7340
                     /label=gRNA Protospacer
                     /note="color: black; sequence: TGGTGCTAGATGCTGCTGTC; added:
                     2023-09-28"
     misc_feature    7338
                     /label=SNV
                     /note="WT = G
                     SNV = T"
                     /note="color: #ff6600"
     misc_feature    7341..7343
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(7530..7549)
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: TCTCCCCAACATCCTACAGC; added:
                     2023-09-28"
     primer_bind     complement(7546..7569)
                     /label=PCR Reverse
                     /note="color: black; sequence: GCTATAGACACAACTCCCACTCTC; 
                     added: 2023-09-28"
     gene            complement(12824..31690)
                     /label=DDX55
                     /note="gene ENSG00000111364
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(12824..31668)
                     /label=DDX55-201
                     /note="primary transcript ENST00000238146
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(13345..15751)
                     /label=DDX55-203
                     /note="primary transcript ENST00000421670
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(join(28836..13361,1..28))
                     /label=GTF2H3-214
                     /note="primary transcript ENST00000618160
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
     gene            complement(join(28837..13361,1..28))
                     /label=GTF2H3
                     /note="gene ENSG00000111358
                     Protein coding"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 cgaggtgctg tgactcgtgc cagcccccta atctgcggaa gtggagtgcg gggagtgcgc
       61 cggaagaggg gtacggaagt gcgccggaag tggggtgcgg aggtgtgcag cgcgctgtca
      121 gactggctcg caggcggcgc ggccggcgga cccgttcgag acagcgcggg cggctcgggt
      181 cccctggggc tccgcagcag gaggacgcca tggacgacaa gggtaaatca agacaggcca
      241 gggctgctag gctacagcag cgccgttcag gggtcccccc aacctctcac atccaggctg
      301 agctcccgaa gagatttctt ttgaatttca ggctgctccg ctggttgtgt ttccagagtc
      361 acaactaaat ccctcgcgct gctcttgttt tcagggatgc gccctggcag tatctgcagg
      421 catccctccc acacatacgc tccttcccag tgccagtctc cttcctgcct tgttggatct
      481 gtcattaaaa ttagaatgaa taaggcatat ttgatgtgct catctagtat ggttattaag
      541 tgaaaaacaa gggcaaacta atacactttt catgtaaaga aataaagggg agtgggtgga
      601 actccatgta taatatttgt aacatatatg tgtatgtgaa tagacatgta ttagcataga
      661 ctgtctggag gggtgcagga gaaattggca agatggttga ctctgggatc ctctcagatt
      721 ttaggaccaa agaggctgaa gagagaatga cttttcatca tcatttactc gggcttaatt
      781 tttaggcctt gcacaccagt agcttttttt tttttttttt tgagacggag tctcgctctg
      841 tcacccaggc tggagtgcag tggcgcagtc tcggctcact gcaagctccg cctcccgggt
      901 tcacgccatt ctcctgcctc agcctcccaa gtagctggga ctacaggcgc ccgccactac
      961 gcccggctaa ttttttgtat ttttagtaga gacggggttt caccgtttta gccgggatgg
     1021 tctcgatctc ctgacctcgt gatccgcccg cctcggactc ccaaagtgct gggattacag
     1081 gcgtgagcca ccgcgcccgg ccaccagtag ctttttcaaa ataaaaaaga gctcttgcaa
     1141 acagcaaagt ggtattttct ctattcccat caacatttat tgcatccctg aaaccaaata
     1201 ccctaaaaag aaagtatttt ttaaattgtt tgcttcttaa agtcaacagt cattaacaag
     1261 ctctcttgcc ttataggctg ctaaatgaat taggtgaatc acttttttcc ctttttccag
     1321 agttaattga atactttaag tctcagatga aagaagatcc tgacatggcc tcagcagtgg
     1381 ctgccatccg gacgttgctg gagttcttga agagagataa aggtatatag agagtcatct
     1441 gttaacactc tgggacttag gatagtcttt tttttctttt cttttttttt ttttttaaag
     1501 agacggagcc tcactctgtc acccaggctg gagtgcagtg gcgagatctt ggctcactgc
     1561 aacctccgcc tcctgggttc aagcaattct cctgccttgg cctcctgagt agctgggatt
     1621 ataggtgtgc accaccttgt ccggctaatt tttttgtatt ttagagacag ggtttcacca
     1681 tgttacccag gctggtctcg aactcctgag cttaggcaat ctgcccgctt tggcctccca
     1741 aagtgctagg attacaggtg tgagccactg tgcccggccg tttgtttttt ttgagacaga
     1801 gttttgctct tgtctcccag gctggagtgc aatggcacaa tctcggctca ctgcaacctc
     1861 tgcctctcaa gttcaagcga ttctcctgcc tcagccttct gagtagctgg gattacaggc
     1921 atgcaccacc acgcctggca aaattttttt tttttttttt gagacggagt ctcgctctgt
     1981 cacccgggct ggagtgcagt ggtgccatct tggctcactg caggctccgc ttcccaggtt
     2041 catgccattc tcctgcctca gcctccaggt agctgggact acaggcgccc gccaccatgc
     2101 ctagctaatt ttttgtattt ttagtagaga cggggtttca ctttgttagc cagaatggtc
     2161 tcgatctcct gacctcgtga tccgcccatg tcggcctccc aaagtgctgg gattgcaggc
     2221 gtgagccacc gggcccggcc aaattttttt atttttagta gagacagggt ttcaccatgt
     2281 tggccacagt ggtcttaaac tcctagcctc aggtgatccg tccgcctcgc cctcccaaag
     2341 tgctgagatt acaggcgtga gccaccatgt tcggccagga aagtcttcta atgactctta
     2401 gaaatgtaga aagtgagaga tgcatgtttg cctgcataaa gtagtaatac agctccaagc
     2461 tcagcttctg ccttcatttc agacctttca atctggagag agctcttacg ttgtcatgaa
     2521 aaggatccct ttcataagtg aaacagactt tgaatcgaga gtgactatgt tatattgtgt
     2581 taaccatgat gcgtaaattt cagtagcagt tcagctttat aagtgtttat taggcctcca
     2641 ccttgtgcca gattctgtac agatgctaca gggaatattc cagaataaat tctggtatta
     2701 gtattattta ttgtgatccc ctaattcagc accctaaatg taagcatctg ggtatagcat
     2761 gaaatcttgt ataagcaaaa taataatgca gcctttaatc tgaagggctt gcctagagac
     2821 acatgtcttt aaggcaacct ccagtgtctt cccattgttc ttaaaataac atccaaacaa
     2881 ccttagcctc acccatacca ccttgcagac ctcatctgcc gctgctactt aatgtctttc
     2941 catgcctcag cctcccaaag tgctgagatt acaggcgtga gccaccatgc ccagccaccc
     3001 agctttattt ctatgtgtgc ttgtttacta tccgcttcct ctattacaat caaaactcca
     3061 tcacagcaga accctacctg tcttgttcac tgatgtatcc ctagcatcta gaatagtgcc
     3121 tggtatatag tatgtaccta gtaaatattc actgaatgaa tgaatgaatt gaagatacag
     3181 gtcggggctg tggccctaat gtgaacatca cattccttgt tggatcattc caggggagac
     3241 aatccagggt ctgagggcga atctcaccag tgccatagaa accctgtgtg gtgtggactc
     3301 ctctgtggca gtgtcctctg gcggggagct cttcctccgc ttcatcagtc ttgcctccct
     3361 ggaatactcc gtaagtgcct gttctccctg gggttacctt tccacttctc tgtttagtga
     3421 cagacattct cagcattaac tggatcttcc ctgtcccctc agtttgctct tttcttccta
     3481 ggattactcc aaatgtaaaa agatcatgat tgagcgggga gaactttttc tcaggagaat
     3541 atcactgtca agaaacaaaa ttgcagatct gtgccatact ttcatcaaag atggagcggt
     3601 gagtggataa tcatcatggt ggtaaggagg agcaacggtt tcgagtccga ctgcctgggg
     3661 taaaatccgg gctctgccac tcacttgctg tggagacttg aacaagtaac ttaacctgtc
     3721 tgtgcagctt tctcatctgt taaataagga tgacaatagt acctacctcc ttgggttgtt
     3781 gtgaggatta aatgaggcaa gacatgtaag gggcaagggg tgtgagggct tggtattgtg
     3841 cctggcactt agtaagtgct gcatgaatgt aggtcttgtt attagagatg gttgtcattg
     3901 taaacaggca gagagggaca gtgggacagc acgtgtgatt cttgatttat tttatttatt
     3961 tatttattta ttttgagacg gagtcttgct ctgtcaccca ggctggagtg cagtggcgcg
     4021 atctcagctc actgcaagct ccgcctcctg ggttcacacc attctcctgc ctcagcctcc
     4081 cgagtagctg ggactacagg tgcccaccag caagcccagc taattttttt tatttttagt
     4141 agagacgggg tttcactgtg ttagccagga tggtctcgat ctcctgacct tgtgatccac
     4201 ccgcttcggc ctcccaaagt gctgggatta caggcgtgag ccaccgcacc tggccctgat
     4261 tcttgattta atacagtgtt ttactaaaga agtattttta tgattgtatg tgtagtaaat
     4321 ctgaattaac agaacagctc ttgtcactgg ttaacactga ggactttctt agtgaaggtt
     4381 agcaatagag ggaaattgag ggaaattttc tttttaactt cattttagat gctattacag
     4441 agatgtaaag ccacgcagtg tttataagtg aggaaagaca ggaggtgcaa gttgaaccat
     4501 cttccagtaa aggtgtaatg aaactatcat aatgcccctc ccaccagcat acgtcctttt
     4561 gccacctagg cctcccctga tcataaaatc atttacttgc attttgactc ttaaatcttg
     4621 tcctccattt tattagcccc ttcccaacct ttgaagtatg ttattaaata aatggcataa
     4681 tagatgtgag tgaaacttgt agaaaagcca gttgcaaacg aagccttttt tgggcaagtg
     4741 gaggcaaggt tgtgacttcc tctcgctggg gccttctcct tccacagacc tgctgtgccg
     4801 tcagtattgt gcgggtttcg tttgctctag tgtagtgtgg tatgtatgta ctttacctgc
     4861 agtcacctac tcaggactga tttagacaaa atttatagca gagctgctca gatttgcaca
     4921 tgcagaggag tcagctgggg tcttattaaa tgcagatcgg gattcagtgg gtttgggcta
     4981 gggtccacga acctgccttc ctaacgggtt ctcaggtgag acccagaaag gagctagaga
     5041 gctggttctg cccccttgtc ttattctgtc ttctgcactc ttccatagaa aagaggttat
     5101 ggaggctggg catggtggct catgcctgta atcccagcac tttggaaagc caagacacgc
     5161 agattgcttg agtccaggag ttcaagacca gcccgggcaa catggcaaaa cttcatctct
     5221 actaaaaata caaaaagtta gccaggcgtg gtggcacact cctgtggtcc cagctacttg
     5281 ggaggctcag gtgggagaat cacctgaacc caggaagttg aggctgcagt gagctgagat
     5341 cataccactg cacttcagcc tgggcaattg gagtgagatc ctgtctcacc aaaaaaaaaa
     5401 aaaaaaaaaa aaaaagaggt tatgggccta ctgtggtggc ttatgtgtat aatcctagcc
     5461 ggaggttccc ttgaggccag gaattcaaca ctagcctgag caacataggg agagaggccc
     5521 tgtctctaca aaaaataaaa taaattagct agtcacagtg gcgcacatct gtagtcccag
     5581 ctactggggt ggctgaggtg ggaggatcgt ttgagcccag gatgtcgagg ctacagtgag
     5641 ccatgaacac tgcactgcac tccagcctag gcatcagagc aagaccttgg ctcaagaaag
     5701 aaaagaaaac agattatgtc taagtgtcta cacattgcat ccacgccaca gataggttgt
     5761 gattacccca aacagtgttt ataaattcaa aaaaattagt tagccatcat ttcaaaattg
     5821 gatttttgaa acatcacctg gtctggcaat cttggggcca catgacgcca gctggggctg
     5881 aggagtcctg gccctcctta gccttttctt tttgagacag agtctcactc tcttgcccag
     5941 actagagtgc actggcatga ttatggctca ctgcagcctc aacctcccag gctcaagtga
     6001 tcctcccacc tcggcctccc aagtagctgg gactataggc acatgccact gcatccagct
     6061 aatttttttt gttgttattt tttgtagaga tgaggtcttg ccaagttgcc cagactggtt
     6121 ttgaactcct gggctcaggc agtcctctca ccttggcctc ccaaaatgct gggattacag
     6181 gcgtgagcca ccacacccag ccttctgtag cctttcgctt tgctgctggc cccacccaac
     6241 ctgctgcagt gacttctgct acctacttgg ttcctgtagg cctgcgtgtg caaaccatgt
     6301 cttagaaata ggaaaaccaa cccaagggaa cctcatggag ccttcagcta tccaggcttt
     6361 tgtatccttt gggtaacact gtgtgggagt tccatttctg cacctgctac aggatgtgag
     6421 acctggcaga aactttttta gcttgcacat gggatggaaa ctgtaccatc aggtctcctt
     6481 tctggttgtg gttgatttgt gagaaagtgt acagagatgc ttagggtgtc gggaacacgt
     6541 gccgcagtgc agagggtgtg agtgagcagc aaggagcctg cccctttgac aaagctttct
     6601 cggtccacag acaatattga ctcacgccta ctccagagtg gtcctgagag tcctggaagc
     6661 agccgtggcg gccaagaagc gatttagtgt atacgtcaca gagtcacagc ctgatttgtc
     6721 agggcaagta cctttctgtt cagttatgtg cccagccata attttacaga gcaaactgcg
     6781 gattacagtc caacaggatc agaaccagaa tgtgccccca gcttgccagc agtcagcact
     6841 gcctttcatt gtaccttttc ctgcctttgg aagaaaaatc actgaatttg cagcaggccg
     6901 cagtatatga tctaggggtt tcacttaagt agttaactcc gtggaagagc agagattgta
     6961 ttgatgaaga tttttatcaa ggaataggta acttatatct ccctctctct agacgataat
     7021 caaagcaaaa cttcctcctt ttttcctgaa tttacatctc ttacccagag tctaagagta
     7081 tagttttcca aaaatacttc tttctaaaga actcaaattc ttgtttcctt agtccaaagt
     7141 attgggatct gccccttaac ccattagtat taatgtgagt ttcaagcaaa catccactgt
     7201 ccacattagg tgacatacat cactgtggta ctgtcttgta ctttctctaa cgttctcaaa
     7261 atgtcatctt cagtaagaaa atggccaaag ccctctgcca cctcaacgtc cctgtcactg
     7321 tggtgctaga tgctgctgtc gggtgagtgc ccatcttccc cagccataat cacctccccc
     7381 accccatgct ggcttacagc aagttttcag attgaaagtt tgagtcccgt tcataaagcc
     7441 acaccaggta gcgtcctact tcttaattaa tagctgccct aaaccagcaa gcttgttatg
     7501 aggttgtgat agcatacaaa gaagacagag ctgtaggatg ttggggagag tgggagttgt
     7561 gtctatagct ttatggctgt aagtgttgag tttgttgttg ttgttgtttt tgtttgtttg
     7621 ttttttgttt ttttgagatg gagtcttgcc ctgttgccca ggctggagtg caatggcatg
     7681 atcttggttc actgcaatct ccacctccca ggttcaagcg attctcctgc ctcagcctcc
     7741 taagtagctg ggattaccgg cgcatgccac catgccacgt taattctttt atatttttag
     7801 tagagatggg gtttcaccat gttggccagg ctggtctcga actcctgaac tcaggtgatc
     7861 tgcccgcctt ggcctcccaa agtgctggga ttacaggcgt gagccaccac gcccagccag
     7921 tgcaaatgca ttttaagagc aaagtacaaa ggtcagttgg gatcagcaga gggtaatcag
     7981 ggctctgtat ttctgtgaag acctggctgt cctcagtggc atactgggat gctttgtctc
     8041 acctagaaga tgcagcttgt gtcgcaaagc caagtcacat gttcctggga ttgtcagtgt
     8101 atttcccaag aaaaaacaag agtagtgatc tcttggaatg aaaacaagat agatgattaa
     8161 ggaaattagg aatgtgttca aaagtaagga ttttgaggaa ttctactaat tgctcatttt
     8221 aaagactaaa ttggccttca tcctaccaga aagattctta aggttgggca cagtggctca
     8281 tacctgaggc aggtggatcg ctagagttca ggagttggag accagcctgg gcaatatggc
     8341 gaaaccccat ctctagggca tggtggcata tgctgtggtc ccagctacct gggaggctga
     8401 aatgggagga tcgcttgagc ctaagaggta gaggtttggg ctgagatcat gccactgcac
     8461 tctagcctgg gcaacagagt gagatctggt ctcaaaaaaa aaaagaaaga tttttaaaac
     8521 tgcataacac ttttgtccaa cttcagatct catcagattt taaaattttg tttggtttgt
     8581 gctgaagtag aaaagaaagt agaggatacc acacttggaa ggaagactgc tgtgtcagga
     8641 cattcttgct tcacttacac acatatgtac gtgtgcatgc gccgggcggc agtgtgaagt
     8701 gtatgtctca ttgtagggtc atgaccagaa aacacccctg gtaacaatgc caggggtcta
     8761 tcgcgtgtta agtgcgcttt aaaaaaaaca ggtagttatg ggaggaactt accttgatag
     8821 aaatggaata ttgatctttg cagatgatga tgctactcgt tagagccaag aaaataaagc
     8881 atgaaaagct ttttttcccc ttagctacat catggagaaa gcagatcttg tcatagttgg
     8941 tgctgaagga gttgttgaaa acggaggaat tattaacaag gtaagagcat cagttctccc
     9001 cagttccctg ctcaagactt ggaggtgtct tgctgaccta ggacaccaca gcagtgttga
     9061 ttatggttcc ttctaatcct gcttcacttc aggacttcat catggtattg agttgttgcc
     9121 catttcccgt aagtcatagc aatgggcagt ccagtttcag tcaaatcttt tttagattga
     9181 tctacataaa tgaagacaat gtaatttggt caggtgtgat gccttatacc tgtaatccta
     9241 gcaatttgag aggccagggt gggaggatca cttgaggcct tgagttcaag accagcctgg
     9301 gcaacatagt gaaaccctgt ctctacaaaa aattagaaaa attagctggg cgtggtggtg
     9361 cttgcctgta gttccagctg cccaggaagc tgaggcagga gtatcacgtg agcccaggaa
     9421 tttgaggttg cagtaagtta tgatcacact actgcattcc agcctgggcg atggtgagac
     9481 cctgtctcag taaaaaaaaa aaaaaaaaaa gtgtaattta tatttataca aaattggctt
     9541 gaattggaat tggggtttgt ctggtttttg ctttatttat gtgtatatgt atgttttatt
     9601 tatgcatata tatacacaca cataatatat atacacaaat atttttaaaa tattatttat
     9661 atatttacat ataaagtatg tatgtataaa atacgtgtat ttatatataa atattatata
     9721 tattttaaaa tagagacaca ttcactctgt tgcccaggct gtagtgtagt acagtgatca
     9781 tagcttactg cagtcttgaa ctcctagcct gaagcaatct ttccacttcc caaagtgcta
     9841 ggattacagg catgagccac tgtggccggc ctgtcatttg ttttcatttt aattgagcac
     9901 atttgacctc aatttattac aatctctctc tccattattt aatttagaaa agcttcatga
     9961 aagctttttt tagttaaaat atatgtaaca taaggccggg cgtagtagct cacgcctata
    10021 atcccagcac tttgggaggc tgaggcaggt agatcatctg aggtcaggag ttcgagacca
    10081 gcctggccaa catggtgaaa ccccatatct actaaaaata caaaaaatta gctgggcatg
    10141 gtggcgggtg cctgtaatcc cagctacttg gcaggctgac gcaggagaat cgcttgaacc
    10201 tgggaggcgg aggtctgcag gcagagtgtt gagtcacatg gtaactatat atttaacttt
    10261 cagaaactaa tgattttcca cagcagctgc actactttac attcccacta gcaaagcaca
    10321 aaggttccaa tttctctaca tccttgccaa cacttgtttt tttaaatttt tttatttttt
    10381 gagatggagt ttcactcttg ctgtccaggc tggagtgcaa tggcacagtc ttggctcact
    10441 gcaacctcca gtgcccgggt tcaagcgagt ctcctgcttc agcctcccta gtagctggga
    10501 ttacaggcac cccccatccc accatgccca gctaattttt tgtattttta gtagagatgg
    10561 ggtttcccta tattggccag ctggtctcaa actcctgacc tcaggtgatc cacccacctc
    10621 ggcctcccaa agtgctggga ttacaggcgt gagccaccac atccagcctc tagtggctgt
    10681 ttctatatcc tttgagaaac atttattttt ttgattccaa agtcagaatt agatatatct
    10741 aatatatgta ttagatatat gtatatctat attatataca taactttcta ttgctgtttt
    10801 ttaattgttc ctttattgta attgagacga ggtctcaccg tgttgcctag gctggtccca
    10861 aactcatggg ttcaggtgat ccacccacct cggcctccca aaatgctgga atacaggcat
    10921 gcaccactgt gcccggcccg cttttctgtg gcttctactt cagatgcact ctagagctca
    10981 tccattccat tttctgcctt cccagattgg aaccaaccag atggctgtgt gtgccaaagc
    11041 acagaacaaa cctttctatg tggttgcaga aagtttcaag tttgtccggc tctttccact
    11101 aaaccagcaa gacgtcccag ataagtttaa ggcaagagtt ttacatattt gaaggggtac
    11161 taaagtctaa acagaaacct ttggaaattg tagtcctaag aatgttccag taattttttc
    11221 acttcctatt gctactttcc ccaacacttg gaatacaaag ataaattggt gattggactc
    11281 acacatttgt ccctagaact tcagtctttc agtctcttta agtagcacca tagatgggtg
    11341 gagaggaagc ccgaagctgg tgggtaagtc ccagctttgg gcttaactga gctcagctcc
    11401 catcaccaca acttacaaac tgtgcgagtt gaggcaagtc ctttcatttc aacctctgaa
    11461 ccggttttct tgtctgtaaa atggcatagc agtatctgac agggtcatga ggattctaag
    11521 ataatcttag cacagtccct ggcacgctgc aagctctcta ttgtgactct cgatcttccc
    11581 ttgagaggtc tggagaagtt aatccggcat gagaatgagg ggacatggga tttaagtttg
    11641 ttacactaca gctctcagcc agcacactgc catgatctgg ctcatggcca caactctgcc
    11701 ctcgtctcta gtgtcctgca tagcttagtt tctcccattt tcagagagca gtctcaggaa
    11761 gtagcacact gatcactcac accaaggccc taccaggtca gagcactaaa tattcagtgc
    11821 cgactaatgt gtacttctct cctcagtata aggcagacac tctcaaggtc gcgcagactg
    11881 gacaagacct caaagaggag catccgtggg tcgactacac tgccccttcc ttaatcactc
    11941 tgctgtttac agacctgggc gtgctgacac cctcagcagt cagcgatgag ctcatcaagc
    12001 tctatctgta acctgtgagc cctttcctgc caaggtgcag cttacgtagt tgaggcaggg
    12061 tgagtagctg cttgacaccc cagtgagtca ggccaaaact gagatgtgtt taatgaagat
    12121 ttatggagta aggacttaaa atcatacatc ttggagaacc tttcttactc atttcagtcc
    12181 catctaaaaa tgtgtcagct attctaaatc ccaacttaaa ttgttcttac ggtttctaga
    12241 aactttcctt ttcagtttcc agaaatacaa gttagataat tggctactta actgatgaaa
    12301 gatgagccca agtccacctg tcttcatcct cccctgcact ccagactgat ctgcctaaag
    12361 cacgcgagat gcaggcgaaa agcagccaca cccctctgcc acaaatgacc aacagctggt
    12421 caggacgtta cacgcggtgc cttgtaagag gcaagaaaca cttgctgaat ctgcatctgg
    12481 cttccagtgg taagcacatt cctcagcagg atcaagccaa acagtaaaaa ctaccaagag
    12541 aacacgagga aggcagaaac gatgtttagc aacagtattc tgcatggttc actgcttaag
    12601 aaaatgcctt ctggaatatt tgtaaactga aattctgtat gtgtaagaga tgtttaaata
    12661 tgtttgggtc ctaaacagct ttttaaaatt atacttggga ataaattcag catcttttca
    12721 aataaactct atgaaagacg ctgatgtggt gttgcatcaa tactgcaagc tgaccaggcc
    12781 atctagggga aaacactgtc cagctacatt tacacactta agacccaggc aatcaggaca
    12841 ccatttattc aacaggaggc ataaaaacta taattctctt tcccggaata tgacttaatc
    12901 cctctgtttt tcagctattc accaataaaa caatactgtg actattaaca cagaaaaacc
    12961 ccagcacatt agccacccct gcaggacatc aaaggataac tgaagcccag ggccaagcac
    13021 attcaggagg tcaaatgcct gaagatactg aggaatggtt ttgaaaatca gaagtcaaga
    13081 agacttctgt atctttatgt ttaaaaataa ctataaaata gcctcagaaa aaagagctta
    13141 tatatatata tatatatata tatatatata tatatatata tatataatat gtacatatgt
    13201 ctatatgtga cccatcatga taaatactga catctattat ttacactcaa ataattttac
    13261 attttcttct gttagactac tccaagactg gaattgtttt gttttgttgt ttttaatgag
    13321 agggtctcgc tgtgttccca ggctggtctt gaactcctgg g
//
